|Positive WB detected in||A549 cells, NIH/3T3 cells, HEK-293 cells, rat kidney tissue, RAW 264.7 cells, HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, RAW 264.7 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15816-1-AP targets PRDX1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PRDX1 fusion protein Ag8602|
|Full Name||peroxiredoxin 1|
|Calculated molecular weight||199 aa, 22 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC007063|
|Gene ID (NCBI)||5052|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (PMID: 9497357). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).
|Product Specific Protocols|
|WB protocol for PRDX1 antibody 15816-1-AP||Download protocol|
|IHC protocol for PRDX1 antibody 15816-1-AP||Download protocol|
|IF protocol for PRDX1 antibody 15816-1-AP||Download protocol|
|FC protocol for PRDX1 antibody 15816-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (03-12-2020)
PRDX1 is easily detected in mouse hearts. It has strong, and clear band.