Validation Data Gallery
|Positive WB detected in||A549 cells, HEK-293 cells, U2OS cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue|
|Positive IHC detected in||human breast cancer tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, RAW 264.7 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15816-1-AP targets PRDX1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PRDX1 fusion protein Ag8602|
|Full Name||peroxiredoxin 1|
|Calculated molecular weight||199 aa, 22 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC007063|
|Gene ID (NCBI)||5052|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (PMID: 9497357). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).
|Product Specific Protocols|
|WB protocol for PRDX1 antibody 15816-1-AP||Download protocol|
|IHC protocol for PRDX1 antibody 15816-1-AP||Download protocol|
|IF protocol for PRDX1 antibody 15816-1-AP||Download protocol|
|FC protocol for PRDX1 antibody 15816-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.
Br J Pharmacol
Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.
Cell Chem Biol
Parvifoline AA Promotes Susceptibility of Hepatocarcinoma to Natural Killer Cell-Mediated Cytolysis by Targeting Peroxiredoxin.
The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.
A novel cuproptosis pattern and tumor immune microenvironment characterization in urothelial carcinoma of the bladder
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guillermo (Verified Customer) (07-20-2022)
Very nice product.
Wei (Verified Customer) (03-12-2020)
PRDX1 is easily detected in mouse hearts. It has strong, and clear band.