PRDX1 Polyclonal antibody

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  WB analysis using 15816-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15816-1-AP (PRDX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 15816-1-AP

  U2OS (WT and PRDX1 KO) lysates prepared with RIPA buffer, 10 μg protein loaded. 15816-1-AP incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HEK-293 using 15816-1-AP

  WB result of PRDX1 antibody (15816-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX1 transfected HEK-293 cells.

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  IHC staining of human breast cancer using 15816-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15816-1-AP (PRDX1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15816-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15816-1-AP (PRDX1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15816-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15816-1-AP (PRDX1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15816-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRDX1 antibody (15816-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PRDX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15816-1-AP

Publications(42)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

PAGA, PAG, NKEF-A, Natural killer cell-enhancing factor A, MSP23

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, U2OS cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 36 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

15816-1-AP targets PRDX1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRDX1 fusion protein Ag8602
• Full Name: peroxiredoxin 1
• Calculated Molecular Weight: 199 aa, 22 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC007063
• Gene Symbol: PRDX1
• Gene ID (NCBI): 5052
• RRID: AB_2170318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q06830
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX1 (Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (PMID: 9497357). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).

Publications

Species	Application	Title
human	WB	Nat Commun; Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.; Authors - Haohao Huang
mouse	WB	Redox Biol; PMID: 32604037; Authors - Anindya Roy Chowdhury
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
		Cell Chem Biol; Parvifoline AA Promotes Susceptibility of Hepatocarcinoma to Natural Killer Cell-Mediated Cytolysis by Targeting Peroxiredoxin.; Authors - Huifang Zhu
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H
rat	WB	Elife; Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control; Authors - Meiling Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guillermo (Verified Customer) (07-20-2022)

Very nice product.

• Applications: Western Blot

FH

Wei (Verified Customer) (03-12-2020)

PRDX1 is easily detected in mouse hearts. It has strong, and clear band.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart