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  • KD/KO Validated

PRDX1 Polyclonal antibody

PRDX1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15816-1-AP

Synonyms

MSP23, NKEF A, NKEFA, PAG, PAGA, PAGB, peroxiredoxin 1, PRDX1, PRX1, PRXI, TDPX2, Thioredoxin peroxidase 2



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, RAW 264.7 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15816-1-AP targets PRDX1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX1 fusion protein Ag8602
Full Name peroxiredoxin 1
Calculated molecular weight 199 aa, 22 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC007063
Gene symbol PRDX1
Gene ID (NCBI) 5052
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (PMID: 9497357). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).

Protocols

Product Specific Protocols
WB protocol for PRDX1 antibody 15816-1-APDownload protocol
IHC protocol for PRDX1 antibody 15816-1-APDownload protocol
IF protocol for PRDX1 antibody 15816-1-APDownload protocol
FC protocol for PRDX1 antibody 15816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.

Authors - Haohao Huang
mouseWB

Redox Biol

PMID: 32604037

Authors - Anindya Roy Chowdhury
mouseWB

Cell Rep

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Authors - Wu Hongtan H
humanWB

Antioxid Redox Signal

Direct Proteomic Mapping of Cysteine Persulfidation.

Authors - Ling Fu
humanWB

Free Radic Biol Med

Oxidative stress as a candidate mechanism for accelerated neuroectodermal differentiation due to trisomy 21.

Authors - Kendra M Prutton
humanWB

Free Radic Biol Med

Maneb alters central carbon metabolism and thiol redox status in a toxicant model of Parkinson's disease.

Authors - Colin C Anderson

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Guillermo (Verified Customer) (07-20-2022)

Very nice product.

  • Applications: Western Blot
FH

Wei (Verified Customer) (03-12-2020)

PRDX1 is easily detected in mouse hearts. It has strong, and clear band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart