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AMPK alpha 2 Antibody

AMPK alpha 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18167-1-AP

Synonyms

AMPK, AMPK alpha 2, AMPK subunit alpha 2, AMPK2, AMPKA2, AMPKα2, PRKAA, PRKAA2



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human skeletal muscle tissue, rat ovary tissue, SKOV-3 cells, MCF-7 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman breast cancer tissue, mouse skeletal muscle tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The 18167-1-AP immunogen is Fusion Protein AMPK alpha 2 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK alpha 2 fusion protein Ag12796
Full Name protein kinase, AMP-activated, alpha 2 catalytic subunit
Calculated molecular weight 552aa,62 kDa
Observed molecular weight 62 kDa
GenBank accession number BC069680
Gene symbol PRKAA2
Gene ID (NCBI) 5563
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKAA2(protein kinase, AMP-activated, alpha 2 catalytic subunit), also named as AMPKA2, AMPK, PRKAA, AMPK2, belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. PRKAA2 is an αβγ heterotrimer that is activated by low cellular energy status, such as decreases in both the ATP/AMP ratio and the phosphocreatine content and it is a glycogen synthase kinase, phosphorylating Ser7 at the NH2 terminus, which decreases glycogen synthase activity (PMID:14532170). The protein can be ubiquitinated (PMID:21224036).

Protocols

Product Specific Protocols
WB protocol for AMPK alpha 2 antibody 18167-1-APDownload protocol
IHC protocol for AMPK alpha 2 antibody 18167-1-APDownload protocol
IF protocol for AMPK alpha 2 antibody 18167-1-APDownload protocol
IP protocol for AMPK alpha 2 antibody 18167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

UBE2O promotes the proliferation, EMT and stemness properties of breast cancer cells through the UBE2O/AMPKα2/mTORC1-MYC positive feedback loop.

Authors - Xu Liu
mouseWB

J Pharmacol Sci

PD149163 induces hypothermia to protect against brain injury in acute cerebral ischemic rats.

Authors - Teng-Fei Xue
humanWB

Lipids Health Dis

Hepatitis C virus nonstructural protein 5A perturbs lipid metabolism by modulating AMPK/SREBP-1c signaling.

Authors - Ziyu Meng
ratWB

Eur J Pharmacol

Activation of AMPK α2 inhibits airway smooth muscle cells proliferation.

Authors - Lu Liu
ratWB

Stem Cells Dev

Normothermic Machine Perfusion Combined with Bone Marrow Mesenchymal Stem Cells Improves the Oxidative Stress Response and Mitochondrial Function in Rat Donation After Circulatory Death Livers.

Authors - Liu Yang
ratWB

Int J Mol Sci

Knockdown of AMPKα2 Promotes Pulmonary Arterial Smooth Muscle Cells Proliferation via mTOR/Skp2/p27(Kip1) Signaling Pathway.

Authors - Rui Ke
  • KD Validated