|Positive WB detected in||HeLa cells, HEK-293 cells, human skeletal muscle tissue, rat ovary tissue, SKOV-3 cells, MCF-7 cells|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human breast cancer tissue, mouse skeletal muscle tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18167-1-AP is AMPK Alpha 2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AMPK Alpha 2 fusion protein Ag12796|
|Full Name||protein kinase, AMP-activated, alpha 2 catalytic subunit|
|Calculated molecular weight||552 aa, 62 kDa|
|Observed molecular weight||62 kDa|
|GenBank accession number||BC069680|
|Gene ID (NCBI)||5563|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PRKAA2(protein kinase, AMP-activated, alpha 2 catalytic subunit), also named as AMPKA2, AMPK, PRKAA, AMPK2, belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. PRKAA2 is an αβγ heterotrimer that is activated by low cellular energy status, such as decreases in both the ATP/AMP ratio and the phosphocreatine content and it is a glycogen synthase kinase, phosphorylating Ser7 at the NH2 terminus, which decreases glycogen synthase activity (PMID:14532170). The protein can be ubiquitinated (PMID:21224036).
|Product Specific Protocols|
|WB protocol for AMPK Alpha 2 antibody 18167-1-AP||Download protocol|
|IHC protocol for AMPK Alpha 2 antibody 18167-1-AP||Download protocol|
|IF protocol for AMPK Alpha 2 antibody 18167-1-AP||Download protocol|
|IP protocol for AMPK Alpha 2 antibody 18167-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
UBE2O promotes the proliferation, EMT and stemness properties of breast cancer cells through the UBE2O/AMPKα2/mTORC1-MYC positive feedback loop.
J Pharmacol Sci
PD149163 induces hypothermia to protect against brain injury in acute cerebral ischemic rats.
Lipids Health Dis
Hepatitis C virus nonstructural protein 5A perturbs lipid metabolism by modulating AMPK/SREBP-1c signaling.
Eur J Pharmacol
Activation of AMPK α2 inhibits airway smooth muscle cells proliferation.
Stem Cells Dev
Normothermic Machine Perfusion Combined with Bone Marrow Mesenchymal Stem Cells Improves the Oxidative Stress Response and Mitochondrial Function in Rat Donation After Circulatory Death Livers.
Int J Mol Sci
Knockdown of AMPKα2 Promotes Pulmonary Arterial Smooth Muscle Cells Proliferation via mTOR/Skp2/p27(Kip1) Signaling Pathway.