Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells, human skeletal muscle tissue, rat ovary tissue, SKOV-3 cells, MCF-7 cells|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human breast cancer tissue, mouse skeletal muscle tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18167-1-AP is AMPK Alpha 2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AMPK Alpha 2 fusion protein Ag12796|
|Full Name||protein kinase, AMP-activated, alpha 2 catalytic subunit|
|Calculated molecular weight||552 aa, 62 kDa|
|Observed molecular weight||62 kDa|
|GenBank accession number||BC069680|
|Gene ID (NCBI)||5563|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PRKAA2(protein kinase, AMP-activated, alpha 2 catalytic subunit), also named as AMPKA2, AMPK, PRKAA, AMPK2, belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. PRKAA2 is an αβγ heterotrimer that is activated by low cellular energy status, such as decreases in both the ATP/AMP ratio and the phosphocreatine content and it is a glycogen synthase kinase, phosphorylating Ser7 at the NH2 terminus, which decreases glycogen synthase activity (PMID:14532170). The protein can be ubiquitinated (PMID:21224036).
|Product Specific Protocols|
|WB protocol for AMPK Alpha 2 antibody 18167-1-AP||Download protocol|
|IHC protocol for AMPK Alpha 2 antibody 18167-1-AP||Download protocol|
|IF protocol for AMPK Alpha 2 antibody 18167-1-AP||Download protocol|
|IP protocol for AMPK Alpha 2 antibody 18167-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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