PKC Delta Polyclonal antibody

PKC Delta Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14188-1-AP

Synonyms

MAY1, PKC delta, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Sample-dependent, check data in validation data gallery

Product Information

14188-1-AP targets PKC Delta in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Delta fusion protein Ag5473
Full Name protein kinase C, delta
Calculated molecular weight 78 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC043350
Gene symbol PRKCD
Gene ID (NCBI) 5580
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKC Delta is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrom.

Protocols

Product Specific Protocols
WB protocol for PKC Delta antibody 14188-1-APDownload protocol
IP protocol for PKC Delta antibody 14188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Chem Biol Interact

ZCL-082, a boron-containing compound, induces apoptosis of non-Hodgkin's lymphoma via targeting p90 ribosomal S6 kinase 1/NF-κB signaling pathway.

Authors - Chunmin Ma
human,mouseWB,IHC

Food Funct

Theaflavin-3,3'-di-gallate represses prostate cancer by activating the PKCδ/aSMase signaling pathway through a 67 kDa laminin receptor.

Authors - Lingli Sun
humanWB

J Biol Chem

Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double strand break repair.

Authors - Trisiani Affandi
mouseWB

JCI Insight

Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy.

Authors - Hongyi Zhou
humanWB

Nat Commun

Discovery of a signaling feedback circuit that defines interferon responses in myeloproliferative neoplasms.

Authors - Diana Saleiro
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wassim (Verified Customer) (04-23-2019)

This antibody picks a band ~76 KDa in mouse kidney tubular cells lysed in RIPA buffer.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Kidney Epithelial Cells
PKC Delta Antibody Western Blot, validation (1:3000 dilution) in Mouse Kidney Epithelial Cells (Cat no:14188-1-AP)
FH

Angela (Verified Customer) (12-17-2018)

Lanes are as follows: 1 SCR control2 pkcd shrna13 pkcd shrna24 pkcd shrna35 50uM etoposide 18hr6 untreated control

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Salivary cell line
PKC Delta Antibody Western Blot, validation (1:1000 dilution) in Salivary cell line (Cat no:14188-1-AP)