Tested Applications
| Positive WB detected in | NIH/3T3 cells, C6 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human ovary cancer tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Saos-2 cells, A431 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14188-1-AP targets PKC Delta in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5473 Product name: Recombinant human PRKCD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 326-676 aa of BC043350 Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED Predict reactive species |
| Full Name | protein kinase C, delta |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC043350 |
| Gene Symbol | PKC Delta |
| Gene ID (NCBI) | 5580 |
| RRID | AB_10638614 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q05655 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PKC Delta is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrom.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PKC Delta antibody 14188-1-AP | Download protocol |
| IHC protocol for PKC Delta antibody 14188-1-AP | Download protocol |
| IP protocol for PKC Delta antibody 14188-1-AP | Download protocol |
| WB protocol for PKC Delta antibody 14188-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PKC Delta Polyclonal antibody (catalog #14188-1-AP) has 18 citations. These appear in journals including Nature Communications, Journal of Advanced Research, Advanced Science, Cell Death & Differentiation, Burns & Trauma, Environmental Research, JCI Insight, Ecotoxicology and Environmental Safety, Food & Function, Molecular Cancer Research, Chemical & Biological Interactions, Cancers, FASEB Journal, Journal of Biological Chemistry, Immunohorizons, and bioRxiv. Research fields span cancer, kidney disease, vascular injury, spinal cord injury, lipodystrophy, hepatic toxicity, radiation protection, and immune signaling.
| Species | Application | Title |
|---|---|---|
Nat Commun Discovery of a signaling feedback circuit that defines interferon responses in myeloproliferative neoplasms.
| ||
J Adv Res Podocyte TLR4 deletion alleviates diabetic kidney disease through prohibiting PKCδ/SHP-1-dependent ER stress and relieving podocyte damage and inflammation | ||
Adv Sci (Weinh) SIRT6 Lysine-Demyristoylates ATF2 to Ameliorate Vascular Injury via PRKCD/VE-Cadherin Pathway Regulating Vascular Endothelial Barrier. | ||
Cell Death Differ SUMOylation-stabilized G6PD orchestrates metabolic rewiring for oxidative stress survival and chemoresistance in HCC via a PKCδ-phosphorylation trigger | ||
Burns Trauma Electroacupuncture reduces calcium influx and inhibits endoplasmic reticulum stress by inhibiting the PKCδ-TRPA1 pathway to promote motor function recovery after spinal cord injury. | ||
Environ Res Effects of PFOA and emerging alternatives at environmental concentrations on murine- and human-derived microglia: A comparative study. |
Reviews
What customers say
Both reviewers rated the antibody 5 stars for Western Blot. One user detected a clear ~76 KDa band in mouse kidney tubular cell lysates (RIPA buffer) at a 1:3000 dilution. The other validated PKD knockdown in a salivary cell line at 1:1000 dilution, showing effective target reduction with shRNA and a stress-response band with etoposide treatment. Overall, the antibody is reported to perform reliably with specific band detection and appropriate dilution ranges for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wassim (Verified Customer) (04-23-2019) | This antibody picks a band ~76 KDa in mouse kidney tubular cells lysed in RIPA buffer.
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Angela (Verified Customer) (12-17-2018) | Lanes are as follows: 1 SCR control2 pkcd shrna13 pkcd shrna24 pkcd shrna35 50uM etoposide 18hr6 untreated control
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