PKC Delta Polyclonal antibody

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  WB analysis using 14188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14188-1-AP (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14188-1-AP

  IP result of anti-PKC Delta (IP:14188-1-AP, 4ug; Detection:14188-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of rat stomach using 14188-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14188-1-AP (PKC Delta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 14188-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14188-1-AP (PKC Delta antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of Saos-2 using 14188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of A431 using 14188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

PKC Delta Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14188-1-AP

Publications(11)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PRKCD, EC:2.7.10.2, EC:2.7.11.13, MAY1, nPKC-delta

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, C6 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human ovary cancer tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Saos-2 cells, A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14188-1-AP targets PKC Delta in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5473

  Product name: Recombinant human PRKCD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 326-676 aa of BC043350

  Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED

• Full Name: protein kinase C, delta
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC043350
• Gene Symbol: PKC Delta
• Gene ID (NCBI): 5580
• RRID: AB_10638614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05655
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKC Delta is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrom.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Discovery of a signaling feedback circuit that defines interferon responses in myeloproliferative neoplasms.; Authors - Diana Saleiro ; KD Validated
mouse	WB	JCI Insight; Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy.; Authors - Hongyi Zhou
rat	WB	Mol Cancer Res; PKCð regulates chromatin remodeling and DNA repair through SIRT6; Authors - Trisiani Affandi
human	WB	J Biol Chem; Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double strand break repair.; Authors - Trisiani Affandi
mouse,human	WB,IHC	Food Funct; Theaflavin-3,3'-di-gallate represses prostate cancer by activating the PKCδ/aSMase signaling pathway through a 67 kDa laminin receptor.; Authors - Lingli Sun
human	WB	Chem Biol Interact; ZCL-082, a boron-containing compound, induces apoptosis of non-Hodgkin's lymphoma via targeting p90 ribosomal S6 kinase 1/NF-κB signaling pathway.; Authors - Chunmin Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wassim (Verified Customer) (04-23-2019)

This antibody picks a band ~76 KDa in mouse kidney tubular cells lysed in RIPA buffer.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Kidney Epithelial Cells

PKC Delta Antibody Western Blot, validation (1:3000 dilution) in Mouse Kidney Epithelial Cells (Cat no:14188-1-AP)

FH

Angela (Verified Customer) (12-17-2018)

Lanes are as follows: 1 SCR control2 pkcd shrna13 pkcd shrna24 pkcd shrna35 50uM etoposide 18hr6 untreated control

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Salivary cell line

PKC Delta Antibody Western Blot, validation (1:1000 dilution) in Salivary cell line (Cat no:14188-1-AP)