DNA-PKcs Polyclonal antibody

DNA-PKcs Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19983-1-AP

Synonyms

DNA PK catalytic subunit, DNAPK, DNA-PKcs, DNPK1, HYRC, HYRC1, p350, p460, PRKDC, XRCC7



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

19983-1-AP targets DNA-PKcs in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein kinase, DNA-activated, catalytic polypeptide
Calculated molecular weight 469 kDa
Observed molecular weight 350-460 kDa
GenBank accession numberNM_006904
Gene symbol PRKDC
Gene ID (NCBI) 5591
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.

Protocols

Product Specific Protocols
WB protocol for DNA-PKcs antibody 19983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Diabetol Metab Syndr

Stem cells from human exfoliated deciduous teeth ameliorate type II diabetic mellitus in Goto-Kakizaki rats.

Authors - Nanquan Rao
humanWB

Cell Death Dis

HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response.

Authors - Zongpei Guo
humanWB

Hum Cell

Flavone protects HBE cells from DNA double-strand breaks caused by PM2.5.

Authors - Xing Ren
humanWB

Int J Biochem Cell Biol

Targeting Ku86 enhances X-ray-induced radiotherapy sensitivity in serous ovarian cancer cells.

Authors - Qian Ma
humanWB

Virology

Interaction of cellular proteins with BCL-xL targeted to cytoplasmic inclusion bodies in adenovirus infected cells.

Authors - T Subramanian
humanWB

Toxicol Appl Pharmacol

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Authors - Hui Dong