|Positive WB detected in||HeLa cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
19983-1-AP targets DNA-PKcs in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||protein kinase, DNA-activated, catalytic polypeptide|
|Calculated molecular weight||469 kDa|
|Observed molecular weight||350-460 kDa|
|GenBank accession number||NM_006904|
|Gene ID (NCBI)||5591|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.
Diabetol Metab Syndr
Stem cells from human exfoliated deciduous teeth ameliorate type II diabetic mellitus in Goto-Kakizaki rats.
Cell Death Dis
HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response.
Flavone protects HBE cells from DNA double-strand breaks caused by PM2.5.
Int J Biochem Cell Biol
Targeting Ku86 enhances X-ray-induced radiotherapy sensitivity in serous ovarian cancer cells.
Interaction of cellular proteins with BCL-xL targeted to cytoplasmic inclusion bodies in adenovirus infected cells.
Toxicol Appl Pharmacol
Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.