Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19983-1-AP targets DNA-PKcs in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein kinase, DNA-activated, catalytic polypeptide |
| Calculated Molecular Weight | 469 kDa |
| Observed Molecular Weight | 350-460 kDa |
| GenBank Accession Number | NM_006904 |
| Gene Symbol | PRKDC |
| Gene ID (NCBI) | 5591 |
| RRID | AB_10642811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78527 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for DNA-PKcs antibody 19983-1-AP | Download protocol |
| WB protocol for DNA-PKcs antibody 19983-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DNA-PKcs Polyclonal antibody (Cat# 19983-1-AP) has 31 citations spanning journals including Science, Nature Communications, Cell Research, Cancer Research, Cell Discovery, Cancer Letters, Phytomedicine, Journal of Cell Biology, and Oncotarget. Research fields center on DNA damage repair (NHEJ/BER/NER), radioresistance and radiosensitization, and cancer biology across glioma, breast, lung, colorectal, pancreatic, bladder, prostate, ovarian, and hepatocellular carcinomas, with additional work in autophagy, immune response, and diabetes.
| Species | Application | Title |
|---|---|---|
Science IFI16 DNA sensor is required for death of lymphoid CD4 T cells abortively infected with HIV. | ||
Cell Res Sensing of DNA double-strand breaks by the NHEJ system stabilizes RORγt transcriptional activity and shapes Th17 pathogenicity in autoimmunity | ||
Cancer Res PGC-1α degradation suppresses mitochondrial biogenesis to confer radiation resistance in glioma | ||
Nat Commun Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. | ||
Cell Death Dis HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response. |
Reviews
What customers say
The product received a 5-star rating from a Western Blot application on mouse heart tissue at a 1:1000 dilution. The reviewer reported strong and correct bands, indicating reliable specificity and performance for their experiment.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
CHAO (Verified Customer) (03-31-2022) | Strong correct bands
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