PRKRIP1 Polyclonal antibody

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  WB analysis using 16794-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16794-1-AP (PRKRIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 16794-1-AP

  IP result of anti-PRKRIP1 (IP:16794-1-AP, 4ug; Detection:16794-1-AP 1:2000) with NIH/3T3 cells lysate 1200 ug.

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  IHC staining of human colon cancer using 16794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16794-1-AP (PRKRIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 16794-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PRKRIP1 antibody (16794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

PRKRIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16794-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C114, PRKR interacting protein 1, PRKR-interacting protein 1

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16794-1-AP targets PRKRIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRKRIP1 fusion protein Ag10266
• Full Name: PRKR interacting protein 1 (IL11 inducible)
• Calculated Molecular Weight: 184 aa, 21 kDa
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: BC014298
• Gene Symbol: PRKRIP1
• Gene ID (NCBI): 79706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H875
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKR-interacting protein 1 (PRKRIP1), a component of the splicing C complex, is a double-stranded RNA-binding protein. PRKRIP1 consists of a fully extended N-terminal loop (residues 51-75) and an 18-turn α helix (residues 76-142) that spans 100 Å (PMID: 28502770). The full-length cDNA contains an open reading frame of 561 bp, which encodes an 184-amino acid polypeptide with a predicted molecular mass of 21 kDa (PMID: 12679338).