|Positive WB detected in
|human testis tissue, mouse testis tissue, rat testis tissue
|Positive IHC detected in
|mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:200-1:1000
|IHC : 1:400-1:1600
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15697-1-AP targets PRM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|PRM1 fusion protein Ag8323
|Calculated molecular weight
|51 aa, 6 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PRM1 is a 51 amino acid protein. PRM1 substitutes for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823)
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chongil (Verified Customer) (08-19-2021)
Prm1 antibody may detect a wrong protein. Prm1 is detected in elongatid spermatids and testicular sperm heads. However, this antibody detected in spermatocytes, round spermatids, and sperm tails but not in sperm heads.