PRM1 Polyclonal antibody

PRM1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15697-1-AP

Synonyms

P1, PRM1



Tested Applications

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

15697-1-AP targets PRM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRM1 fusion protein Ag8323
Full Name PRM1
Calculated molecular weight 51 aa, 6 kDa
Observed molecular weight13-20 kDa
GenBank accession numberBC003673
Gene symbol PRM1
Gene ID (NCBI) 5619
RRIDAB_2878168
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRM1 is a 51 amino acid protein. PRM1 substitutes for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823)

Protocols

Product Specific Protocols
WB protocol for PRM1 antibody 15697-1-APDownload protocol
IHC protocol for PRM1 antibody 15697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Environ Pollut

Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.

Authors - Jianhui Liu
mouseWB

Int J Mol Sci

BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis.

Authors - Chao Huang
mouseWB

Theriogenology

Eif2s3y alleviated LPS-induced damage to mouse testis and maintained spermatogenesis by negatively regulating Adamts5

Authors - Yunxiang Li
mouseWB

Reprod Sci

Sertoli Cell-Conditioned Medium Induces Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells to Male Germ-Like Cells in Busulfan-Induced Azoospermic Mouse Model

Authors - Nasrin Khanmohammadi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chongil (Verified Customer) (08-19-2021)

Prm1 antibody may detect a wrong protein. Prm1 is detected in elongatid spermatids and testicular sperm heads. However, this antibody detected in spermatocytes, round spermatids, and sperm tails but not in sperm heads.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Mouse testis section
PRM1 Antibody Immunofluorescence validation (1:300 dilution) in Mouse testis section (Cat no:15697-1-AP)