PRM1 Polyclonal antibody

PRM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 15697-1-AP

Synonyms

P1, PRM1

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Validation Data Gallery

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  WB analysis using 15697-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15697-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 15697-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 15697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15697-1-AP (PRM1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 15697-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRM1 antibody (15697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

15697-1-AP targets PRM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRM1 fusion protein Ag8323
• Full Name: PRM1
• Calculated Molecular Weight: 51 aa, 6 kDa
• Observed Molecular Weight: 13-20 kDa
• GenBank Accession Number: BC003673
• Gene Symbol: PRM1
• Gene ID (NCBI): 5619
• RRID: AB_2878168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRM1 is a 51 amino acid protein. PRM1 substitutes for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823)

Publications

Species	Application	Title
mouse	WB	Environ Pollut; Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.; Authors - Jianhui Liu
mouse	WB	Int J Mol Sci; BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis.; Authors - Chao Huang
mouse	WB	Theriogenology; Eif2s3y alleviated LPS-induced damage to mouse testis and maintained spermatogenesis by negatively regulating Adamts5; Authors - Yunxiang Li
mouse	WB	Reprod Sci; Sertoli Cell-Conditioned Medium Induces Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells to Male Germ-Like Cells in Busulfan-Induced Azoospermic Mouse Model; Authors - Nasrin Khanmohammadi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chongil (Verified Customer) (08-19-2021)

Prm1 antibody may detect a wrong protein. Prm1 is detected in elongatid spermatids and testicular sperm heads. However, this antibody detected in spermatocytes, round spermatids, and sperm tails but not in sperm heads.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Mouse testis section

PRM1 Antibody Immunofluorescence validation (1:300 dilution) in Mouse testis section (Cat no:15697-1-AP)