PRPF3 Polyclonal antibody

PRPF3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 10106-1-AP

Synonyms

HPRP3, HPRP3P, Pre mRNA splicing factor 3, PRP3, Prp3p, PRPF3, RP18, U4/U6 snRNP 90 kDa protein



Tested Applications

Positive WB detected inY79 cells, HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

10106-1-AP targets PRPF3 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRPF3 fusion protein Ag0154
Full Name PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae)
Calculated molecular weight 78 kDa
Observed molecular weight 90 kDa, 78 kDa
GenBank accession numberBC000184
Gene symbol PRPF3
Gene ID (NCBI) 9129
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3. A predicted molecular mass of 77 kDa and the gene is named PRPF3 because it is homologous to the yeast PRP3 gene. We noticed that although the predicted molecular mass of PRPF3 is77 kDa, the protein produced in E.coli migrated like a 90 kDa protein in SDS-PAGE. (PMID: 9328476)

Protocols

Product Specific Protocols
WB protocol for PRPF3 antibody 10106-1-APDownload protocol
IP protocol for PRPF3 antibody 10106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

PRPF19 regulates p53-dependent cellular senescence by modulating alternative splicing of MDM4 mRNA.

Authors - Kimiyoshi Yano
humanWB

Mol Cell Biol

A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.

Authors - Berg Michael G MG
humanWB

Hum Mol Genet

PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.

Authors - Tanackovic Goranka G