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  • KD/KO Validated

PSAP Polyclonal antibody

PSAP Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10801-1-AP

Synonyms

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1



Tested Applications

Positive WB detected inA431 cells, mouse brain tissue, MCF-7 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10801-1-AP targets PSAP in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSAP fusion protein Ag1194
Full Name prosaposin
Calculated molecular weight 58 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC001503
Gene symbol PSAP
Gene ID (NCBI) 5660
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.

Protocols

Product Specific Protocols
WB protocol for PSAP antibody 10801-1-APDownload protocol
IHC protocol for PSAP antibody 10801-1-APDownload protocol
IF protocol for PSAP antibody 10801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Front Cell Neurosci

PMID: 31143101

Authors - Hassan Saadi
humanWB

Cancer Sci

Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth.

Authors - Wu Yihong Y
humanELISA

Prostate

Serum prosaposin levels are increased in patients with advanced prostate cancer.

Authors - Koochekpour Shahriar S
mouseWB

J Neurochem

Prosaposin in the secretome of marrow stroma-derived neural progenitor cells protects neural cells from apoptotic death.

Authors - Li Na N
mouseWB,IF

J Neurochem

The interaction between progranulin and prosaposin is mediated by granulins and the linker region between saposin B and C.

Authors - Xiaolai Zhou
  • KO Validated
mouseIHC

Proteomics

Proteomic analysis of synaptosomal protein expression reveals that cerebral ischemia alters lysosomal Psap processing.

Authors - Costain Willard J WJ

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sharan (Verified Customer) (05-28-2020)

The antibody works well in western blot assays with minimum cross-reactivity

  • Applications: Western Blot
PSAP Antibody Western Blot validation ( dilution) in (Cat no:10801-1-AP)
FH

Krishnendu (Verified Customer) (02-27-2020)

Very specific for AIMP3

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000 for WB, 1:100 For IP and 1:250 for IF
  • Cell Tissue Type: HEK293T, MCF7