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  • KD/KO Validated

PSAP Polyclonal antibody

PSAP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10801-1-AP

Synonyms

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1



Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, mouse cerebellum tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10801-1-AP targets PSAP in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSAP fusion protein Ag1194
Full Name prosaposin
Calculated molecular weight 58 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC001503
Gene symbol PSAP
Gene ID (NCBI) 5660
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.

Protocols

Product Specific Protocols
WB protocol for PSAP antibody 10801-1-APDownload protocol
IHC protocol for PSAP antibody 10801-1-APDownload protocol
IF protocol for PSAP antibody 10801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,WB

Nat Neurosci

Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis.

Authors - Ruilin Tian
humanWB

Nat Commun

Prosaposin is a regulator of progranulin levels and oligomerization.

Authors - Alexandra M Nicholson
  • KD Validated
humanWB

Nat Commun

Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations.

Authors - Xiaolai Zhou
mouseWB

Mol Neurodegener

Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains.

Authors - Md Golam Sharoar
mouseWB

Proc Natl Acad Sci U S A

Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.

Authors - Constance S Petit
humanWB

Theranostics

Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.

Authors - Mengmeng Qin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sharan (Verified Customer) (05-28-2020)

The antibody works well in western blot assays with minimum cross-reactivity

  • Applications: Western Blot
PSAP Antibody Western Blot validation ( dilution) in (Cat no:10801-1-AP)
FH

Krishnendu (Verified Customer) (02-27-2020)

Very specific for AIMP3

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000 for WB, 1:100 For IP and 1:250 for IF
  • Cell Tissue Type: HEK293T, MCF7