PSAP Polyclonal antibody

• Featured Product
• KD/KO Validated

PSAP Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (2)

Cat no : 10801-1-AP

Synonyms

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

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Validation Data Gallery

• 

  WB analysis using 10801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10801-1-AP (PSAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10801-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10801-1-AP (PSAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10801-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 10801-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10801-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10801-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, mouse brain tissue, MCF-7 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• ELISA: See 1 publications below

Product Information

10801-1-AP targets PSAP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSAP fusion protein Ag1194
• Full Name: prosaposin
• Calculated molecular weight: 58 kDa
• Observed molecular weight: 60 kDa
• GenBank accession number: BC001503
• Gene symbol: PSAP
• Gene ID (NCBI): 5660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.

Publications

Species	Application	Title
mouse	WB,IF	Front Cell Neurosci; PMID: 31143101; Authors - Hassan Saadi
human	WB	Cancer Sci; Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth.; Authors - Wu Yihong Y
human	ELISA	Prostate; Serum prosaposin levels are increased in patients with advanced prostate cancer.; Authors - Koochekpour Shahriar S
mouse	WB	J Neurochem; Prosaposin in the secretome of marrow stroma-derived neural progenitor cells protects neural cells from apoptotic death.; Authors - Li Na N
mouse	WB,IF	J Neurochem; The interaction between progranulin and prosaposin is mediated by granulins and the linker region between saposin B and C.; Authors - Xiaolai Zhou ; KO Validated
mouse	IHC	Proteomics; Proteomic analysis of synaptosomal protein expression reveals that cerebral ischemia alters lysosomal Psap processing.; Authors - Costain Willard J WJ

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sharan (Verified Customer) (05-28-2020)

The antibody works well in western blot assays with minimum cross-reactivity

• Applications: Western Blot

PSAP Antibody Western Blot validation ( dilution) in (Cat no:10801-1-AP)

FH

Krishnendu (Verified Customer) (02-27-2020)

Very specific for AIMP3

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000 for WB, 1:100 For IP and 1:250 for IF
• Cell Tissue Type: HEK293T, MCF7

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