PSAP Polyclonal antibody

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  WB analysis using 10801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10801-1-AP (PSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10801-1-AP

  WB result of PSAP antibody (10801-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

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  IHC staining of human breast cancer using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 10801-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PSAP antibody (10801-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 10801-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSAP antibody (10801-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 10801-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10801-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PSAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10801-1-AP

Publications(22)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Dispersin, CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HeLa cells, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, mouse cerebellum tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HEK-293 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• ELISA: See 2 publications below
• FC: See 1 publications below

Product Information

10801-1-AP targets PSAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSAP fusion protein Ag1194
• Full Name: prosaposin
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC001503
• Gene Symbol: PSAP
• Gene ID (NCBI): 5660
• RRID: AB_2172462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07602
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF,WB	Nat Neurosci; Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis.; Authors - Ruilin Tian
human	IF	Adv Sci (Weinh); Integrative Transcriptomic Analyses of Hippocampal-Entorhinal System Subfields Identify Key Regulators in Alzheimer's Disease; Authors - Dan Luo
human	WB	Nat Commun; Prosaposin is a regulator of progranulin levels and oligomerization.; Authors - Alexandra M Nicholson ; KD Validated
human	WB	Nat Commun; Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations.; Authors - Xiaolai Zhou
mouse	WB	Mol Neurodegener; Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains.; Authors - Md Golam Sharoar
mouse	WB	Proc Natl Acad Sci U S A; Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.; Authors - Constance S Petit

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sharan (Verified Customer) (05-28-2020)

The antibody works well in western blot assays with minimum cross-reactivity

• Applications: Western Blot

PSAP Antibody Western Blot validation ( dilution) in (Cat no:10801-1-AP)

FH

Krishnendu (Verified Customer) (02-27-2020)

Very specific for AIMP3

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000 for WB, 1:100 For IP and 1:250 for IF
• Cell Tissue Type: HEK293T, MCF7