|Positive WB detected in||A431 cells, mouse brain tissue, MCF-7 cells, HeLa cells, HepG2 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10801-1-AP targets PSAP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PSAP fusion protein Ag1194|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC001503|
|Gene ID (NCBI)||5660|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.
Front Cell Neurosci
Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth.
Serum prosaposin levels are increased in patients with advanced prostate cancer.
Prosaposin in the secretome of marrow stroma-derived neural progenitor cells protects neural cells from apoptotic death.
The interaction between progranulin and prosaposin is mediated by granulins and the linker region between saposin B and C.
Proteomic analysis of synaptosomal protein expression reveals that cerebral ischemia alters lysosomal Psap processing.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sharan (Verified Customer) (05-28-2020)
The antibody works well in western blot assays with minimum cross-reactivity
Krishnendu (Verified Customer) (02-27-2020)
Very specific for AIMP3