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  • KD/KO Validated

PSAP Polyclonal antibody

PSAP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (4)





Cat no : 10801-1-AP


A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

Tested Applications

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, mouse cerebellum tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HEK-293 cells, U-251 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10801-1-AP targets PSAP in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, goat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSAP fusion protein Ag1194
Full Name prosaposin
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.


Product Specific Protocols
WB protocol for PSAP antibody 10801-1-APDownload protocol
IHC protocol for PSAP antibody 10801-1-APDownload protocol
IF protocol for PSAP antibody 10801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Neurosci

Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis.

Authors - Ruilin Tian

Adv Sci (Weinh)

Integrative Transcriptomic Analyses of Hippocampal-Entorhinal System Subfields Identify Key Regulators in Alzheimer's Disease

Authors - Dan Luo

Nat Commun

Prosaposin is a regulator of progranulin levels and oligomerization.

Authors - Alexandra M Nicholson
  • KD Validated

Nat Commun

Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations.

Authors - Xiaolai Zhou

Mol Neurodegener

Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains.

Authors - Md Golam Sharoar

Proc Natl Acad Sci U S A

Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.

Authors - Constance S Petit


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sharan (Verified Customer) (05-28-2020)

The antibody works well in western blot assays with minimum cross-reactivity

  • Applications: Western Blot
PSAP Antibody Western Blot validation ( dilution) in (Cat no:10801-1-AP)

Krishnendu (Verified Customer) (02-27-2020)

Very specific for AIMP3

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000 for WB, 1:100 For IP and 1:250 for IF
  • Cell Tissue Type: HEK293T, MCF7