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PSD95-Specific,DLG4 Polyclonal antibody

PSD95-Specific,DLG4 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 20665-1-AP


Disks large homolog 4, DLG4, PSD 95, PSD95, PSD95-Specific,DLG4, SAP 90, SAP90, Synapse associated protein 90

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inNeuro-2a cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20665-1-AP targets PSD95-Specific,DLG4 in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name discs, large homolog 4 (Drosophila)
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90-95 kDa
GenBank Accession NumberNM_001365
Gene Symbol DLG4
Gene ID (NCBI) 1742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).


Product Specific Protocols
WB protocol for PSD95-Specific,DLG4 antibody 20665-1-APDownload protocol
IHC protocol for PSD95-Specific,DLG4 antibody 20665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tatyana (Verified Customer) (03-11-2023)

Works well in IHC and WB in mouse brain. WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~100kDa. IHC: 4% PFA fixed and paraffinized sections; standard antigen retrieval and DAB detection. Used at 1:1000, with incubation overnight at 4C.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse, CNS tissue
PSD95-Specific,DLG4 Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Mouse, CNS tissue (Cat no:20665-1-AP)

Lisa (Verified Customer) (03-17-2022)

The antibody works well for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: hiPSCs-derived neurons

James (Verified Customer) (09-29-2021)

Antibody was not as specific as I had hoped. I used the antibody at 1:1000, I detected a band at 95kDa which is PSD-95, however, other bands were also detected. I will be looking to purchase a more specific monoclonal antibody for my next experiments as the specificity is an issue for me.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Neurogenin 2 iPSC Neurons Day 21
PSD95-Specific,DLG4 Antibody Western Blot validation (1:1000 dilution) in Neurogenin 2 iPSC Neurons Day 21 (Cat no:20665-1-AP)