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PSD95-Specific,DLG4 Polyclonal antibody

PSD95-Specific,DLG4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20665-1-AP

Synonyms

Disks large homolog 4, DLG4, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90



Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, HeLa cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

20665-1-AP targets PSD95-Specific,DLG4 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name discs, large homolog 4 (Drosophila)
Calculated molecular weight 85 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberNM_001365
Gene symbol DLG4
Gene ID (NCBI) 1742
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).

Protocols

Product Specific Protocols
WB protocol for PSD95-Specific,DLG4 antibody 20665-1-APDownload protocol
IHC protocol for PSD95-Specific,DLG4 antibody 20665-1-APDownload protocol
IP protocol for PSD95-Specific,DLG4 antibody 20665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Neural Regen Res

PMID: 30632505

Authors - Xu-Sheng Yan
hamsterWB

Int J Mol Sci

PMID: 30845718

Authors - Syed Zahid Ali Shah
humanWB

J Cell Physiol

The overexpression of GRASP might inhibit cell proliferation and invasion in hepatocellular carcinoma.

Authors - Yang Cheng
mouseWB

J Neurosci

CAMK2-dependent signaling in neurons is essential for survival.

Authors - Martijn J Kool
mouseWB

J Neurosci Res

PMID: 31081159

Authors - Hao Guo
ratWB

Chemosphere

Chronic fluoride exposure induces neuronal apoptosis and impairs neurogenesis and synaptic plasticity: Role of GSK-3β/β-catenin pathway.

Authors - Pei Jiang