|Positive WB detected in||HeLa cells, MCF-7 cells, mouse testis tissue, rat brain tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14786-1-AP targets PSMD11 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PSMD11 fusion protein Ag6435|
|Full Name||proteasome (prosome, macropain) 26S subunit, non-ATPase, 11|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC000437|
|Gene ID (NCBI)||5717|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.
BMC Dev Biol
Generation and identification of a conditional knockout allele for the PSMD11 gene in mice.
J Cell Biochem
Homoharringtonine could induce quick protein synthesis of PSMD11 through activating MEK1/ERK1/2 signaling pathway in pancreatic cancer cells.
Susceptibility of microtubule-associated protein 1 light chain 3β (MAP1LC3B/ LC3B) knockout mice to lung injury and fibrosis.
Mol Cell Proteomics
Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells.
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.