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PSMD11 Polyclonal antibody

PSMD11 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 14786-1-AP

Synonyms

p44.5, PSMD11, Rpn6, S9



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse testis tissue, rat brain tissue
Positive IP detected inMCF-7 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14786-1-AP targets PSMD11 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD11 fusion protein Ag6435
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC000437
Gene symbol PSMD11
Gene ID (NCBI) 5717
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.

Protocols

Product Specific Protocols
WB protocol for PSMD11 antibody 14786-1-APDownload protocol
IF protocol for PSMD11 antibody 14786-1-APDownload protocol
IP protocol for PSMD11 antibody 14786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

BMC Dev Biol

Generation and identification of a conditional knockout allele for the PSMD11 gene in mice.

Authors - Linlin Zhao
humanWB

J Cell Biochem

Homoharringtonine could induce quick protein synthesis of PSMD11 through activating MEK1/ERK1/2 signaling pathway in pancreatic cancer cells.

Authors - Lele Wang
mouseWB

FASEB J

Susceptibility of microtubule-associated protein 1 light chain 3β (MAP1LC3B/ LC3B) knockout mice to lung injury and fibrosis.

Authors - Vidya Sagar Kesireddy
humanWB

Mol Cell Proteomics

Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells.

Authors - Qi Tong-Gang TG
humanWB

Oncogene

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Authors - Sparks A A