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- KD/KO Validated
PTEN Polyclonal antibody
PTEN Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
144
Cat no : 22034-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, NIH/3T3 cells, mouse testis tissue, mouse thymus tissue, HeLa cells, MCF-7 cells, HEK-293T cells, LNCaP cells, Jurkat cells, C6 cells, rat testis tissue |
| Positive IP detected in | DU 145 cells |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue, human testis tissue, mouse brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 17 publications below |
| WB | See 130 publications below |
| IHC | See 27 publications below |
| IF | See 12 publications below |
| IP | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
The immunogen of 22034-1-AP is PTEN Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PTEN fusion protein Ag17274 |
| Full Name | phosphatase and tensin homolog |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 55 kDa, 68 kDa |
| GenBank accession number | BC005821 |
| Gene symbol | PTEN |
| Gene ID (NCBI) | 5728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Phosphatase and tensin homolog (PTEN) is an enzyme key in cell cycle regulation and in tumor suppression.
What is the molecular weight of PTEN?
PTEN is composed of 403 amino acid residues with an N-terminal domain with phosphatase activity and a
C-terminal C2 domain that binds phospholipid membranes (PMID:14749127). This antibody can recognize
two isoforms with MWs of 55kDa and 68 kDa.
What is the function of PTEN?
Widely expressed throughout the body, PTEN is found in the cytoplasm and inhibits cell cycle progression
through phosphatase activity. It regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate (PIP3)
by catalyzing the dephosphorylation of the inositol ring. This function counteracts the growth-stimulating
activity of the PI3K/AKT signaling pathway and thereby suppresses proliferation. Therefore, in homeostasis,
PTEN promotes chromosome stability and DNA repair (PMID: 24387334).Expression and function can be
regulated by posttranslational modifications, including acetylation for decreased catalytic activity and
ubiquitination for protein degradation, nuclear localization, and inhibition of phosphatase activity
(PMID: 24656806).
What is the role of PTEN in cancer?
PTEN was first identified as a tumor suppressor when mutations or deletions were identified by two separate
groups in a number of different cancer types including gliomas, breast, prostate, and kidney cancer
(PMID: 9090379; PMID: 9072974). Non-inherited, or somatic, mutations in PTEN that lead to a loss of function
in the protein have been shown to drive malignancy in tumors. PTEN hamartoma tumor syndrome (PHTS) is a
group of inherited disorders that occur as a result of autosomal dominant mutations in the PTEN gene. These
disorders include Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and Proteus syndrome. Because of
the constitutive expression of PTEN, the symptoms are varied but often include neurodevelopmental issues and
an increased risk of developing tumors, both benign and malignant (PMID: 26564076).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTEN antibody 22034-1-AP | Download protocol |
| IHC protocol for PTEN antibody 22034-1-AP | Download protocol |
| IP protocol for PTEN antibody 22034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomater Res High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration | ||
Drug Des Devel Ther Network Pharmacology and Experimental Validation to Explore That Celastrol Targeting PTEN is the Potential Mechanism of Tripterygium wilfordii (Lév.) Hutch Against IgA Nephropathy | ||
J Pineal Res Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload | ||
Theranostics Histone demethylase JMJD3 protects against renal fibrosis by suppressing TGFβ and Notch signaling and preserving PTEN expression. | ||
Cell Prolif YTHDC1 positively regulates PTEN expression and plays a critical role in cisplatin resistance of bladder cancer | ||
Environ Int The development of a cell-based model for the assessment of carcinogenic potential upon long-term PM2.5 exposure. |
