SHP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SHP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

34

Cat no : 20145-1-AP

Synonyms

PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48

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Validation Data Gallery

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  WB analysis using 20145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20145-1-AP (PTPN11/SHP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20145-1-AP

  IP result of anti-PTPN11/SHP2 (IP:20145-1-AP, 4ug; Detection:20145-1-AP 1:10000) with HEK-293 cells lysate 1575 ug.

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  IHC staining of human breast cancer using 20145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 20145-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 20145-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 20145-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 20145-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20145-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHP2 antibody (20145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human gliomas tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 32 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

20145-1-AP targets SHP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHP2 fusion protein Ag13660
• Full Name: protein tyrosine phosphatase, non-receptor type 11
• Calculated Molecular Weight: 597 aa, 68 kDa
• Observed Molecular Weight: 68-75 kDa
• GenBank Accession Number: BC008692
• Gene Symbol: PTPN11
• Gene ID (NCBI): 5781
• RRID: AB_10699877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nat Commun; Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma; Authors - Perla Pucci
rat	WB	Hepatology; Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.; Authors - Daimin Xiang ; KD Validated
mouse	WB	Oncogene; Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.; Authors - Y Patel
mouse	Cell treatment	Cell Chem Biol; Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells.; Authors - Rukang Zhang
human	WB	Mol Ther Nucleic Acids; miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.; Authors - Tao Han
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu