|Positive WB detected in||A549 cells, Jurkat cells, MCF-7 cells, human heart tissue, human skeletal muscle tissue, human brain tissue, NIH/3T3 cells, HEK-293 cells, U-937 cells, A431 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20145-1-AP is SHP2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SHP2 fusion protein Ag13660|
|Full Name||protein tyrosine phosphatase, non-receptor type 11|
|Calculated molecular weight||597 aa, 68 kDa|
|Observed molecular weight||68-75 kDa|
|GenBank accession number||BC008692|
|Gene ID (NCBI)||5781|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for SHP2 antibody 20145-1-AP||Download protocol|
|IHC protocol for SHP2 antibody 20145-1-AP||Download protocol|
|IF protocol for SHP2 antibody 20145-1-AP||Download protocol|
|IP protocol for SHP2 antibody 20145-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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