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- KD/KO Validated
PTPN11/SHP2 Polyclonal antibody
PTPN11/SHP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, Cell treatment, ELISA
Conjugate
Unconjugated
Cat no : 20145-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, A549 cells, human brain tissue, human heart tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, U-937 cells, HEK-293 cells, HeLa cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human breast cancer tissue, human gliomas tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 27 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
20145-1-AP targets PTPN11/SHP2 in WB, IP, IHC, IF, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PTPN11/SHP2 fusion protein Ag13660 |
Full Name | protein tyrosine phosphatase, non-receptor type 11 |
Calculated molecular weight | 597 aa, 68 kDa |
Observed molecular weight | 68-75 kDa |
GenBank accession number | BC008692 |
Gene symbol | PTPN11 |
Gene ID (NCBI) | 5781 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for PTPN11/SHP2 antibody 20145-1-AP | Download protocol |
IHC protocol for PTPN11/SHP2 antibody 20145-1-AP | Download protocol |
IF protocol for PTPN11/SHP2 antibody 20145-1-AP | Download protocol |
IP protocol for PTPN11/SHP2 antibody 20145-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hepatology Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.
| ||
Oncogene Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis. | ||
Cell Chem Biol Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells. | ||
Mol Ther Nucleic Acids miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance. | ||
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. | ||
Aging (Albany NY) Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells. |