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SHP2 Polyclonal antibody

SHP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20145-1-AP

Synonyms

BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2



Tested Applications

Positive WB detected inA431 cells, A549 cells, human brain tissue, human heart tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, U-937 cells, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human gliomas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20145-1-AP is SHP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHP2 fusion protein Ag13660
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68-75 kDa
GenBank accession numberBC008692
Gene symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SHP2 antibody 20145-1-APDownload protocol
IHC protocol for SHP2 antibody 20145-1-APDownload protocol
IP protocol for SHP2 antibody 20145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neuropsychiatr Dis Treat

The abnormal activation of D1R/Shp-2 complex involved in levodopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson's rats.

Authors - Na Wu
mouseWB

Oncogene

Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Authors - Y Patel
humanWB

Cell Death Dis

Anticancer activity of dietary xanthone α-mangostin against hepatocellular carcinoma by inhibition of STAT3 signaling via stabilization of SHP1.

Authors - Hai Zhang
humanWB

Aging (Albany NY)

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.

Authors - Changying Shi
humanWB,IHC

Oncol Lett

Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma.

Authors - Zhong-Qian Hu
humanWB

Int J Med Sci

PMID: 30008594

Authors - Yangyang He