• Featured Product
  • KD/KO Validated

SHP2 Polyclonal antibody

SHP2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20145-1-AP

Synonyms

BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2



Tested Applications

Positive WB detected inA549 cells, Jurkat cells, MCF-7 cells, human heart tissue, human skeletal muscle tissue, human brain tissue, NIH/3T3 cells, HEK-293 cells, U-937 cells, A431 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20145-1-AP is SHP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHP2 fusion protein Ag13660
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68-75 kDa
GenBank accession numberBC008692
Gene symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SHP2 antibody 20145-1-APDownload protocol
IHC protocol for SHP2 antibody 20145-1-APDownload protocol
IF protocol for SHP2 antibody 20145-1-APDownload protocol
IP protocol for SHP2 antibody 20145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neuropsychiatr Dis Treat

The abnormal activation of D1R/Shp-2 complex involved in levodopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson's rats.

Authors - Na Wu
mouseWB

Oncogene

Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Authors - Y Patel
humanWB

Cell Death Dis

Anticancer activity of dietary xanthone α-mangostin against hepatocellular carcinoma by inhibition of STAT3 signaling via stabilization of SHP1.

Authors - Hai Zhang
humanWB

Aging (Albany NY)

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.

Authors - Changying Shi
humanWB,IHC

Oncol Lett

Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma.

Authors - Zhong-Qian Hu
humanWB

Int J Med Sci

PMID: 30008594

Authors - Yangyang He