Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11586-1-AP targets PUM2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2179 Product name: Recombinant human PUM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-489 aa of BC024218 Sequence: NRFPNLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQMVFNEILQAAYQLMTDVFGNYVIQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISEMVKELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYYLKNSPDLGPIGGPPNGML Predict reactive species |
| Full Name | pumilio homolog 2 (Drosophila) |
| Calculated Molecular Weight | 114 kDa |
| Observed Molecular Weight | 95 kDa, 140 kDa |
| GenBank Accession Number | BC024218 |
| Gene Symbol | PUM2 |
| Gene ID (NCBI) | 23369 |
| RRID | AB_2284639 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TB72 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PUM2 (Human Pumilio homology protein 2), a member of the PUF protein family, binds to 3′ untranslated regions (3′ UTRs) of mRNA and to repress gene expression by controlling cytoplasmic polyadenylation and affecting mRNA translation [PMID:12459267]. When translationally dormant, PUM2, CPEB (CPE-binding protein) and Maskin form a complex on maternal cyclin B1 mRNA, precluding the formation of the active translation initiation complex [PMID:15687499]. PUM2 has been found to play a role in the mammalian nervous system, in particular in regulating morphology, arborization and excitability of neuronal dendrites [PMID:22016787]. The calculated molecular weight of PUM2 is 114 kDa, but the modified PUM2 protein is about 140 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PUM2 antibody 11586-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PUM2 Polyclonal antibody (Cat# 11586-1-AP) has 2 total citations. They appear in Reproduction and Biomedical Pharmacotherapy. The research fields covered include regulation of proliferation and innate immunity in human granulosa tumor cells, and promotion of breast cancer cell stemness via competitive binding to neuropilin-1 mRNA with miR-376a.
| Species | Application | Title |
|---|---|---|
Reproduction The RNA-binding protein Pumilio-2 has roles in the regulation of proliferation and innate immunity of human KGN granulosa tumor cells. | ||
Biomed Pharmacother RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a.
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