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PUMA Polyclonal antibody

PUMA Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 55120-1-AP



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:500
Sample-dependent, check data in validation data gallery

Product Information

55120-1-AP targets PUMA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name BCL2 binding component 3
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberNM_014417
Gene symbol BBC3
Gene ID (NCBI) 27113
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PUMA, also named as JFY-1 and BBC3, belongs to the Bcl-2 family. It is a critical mediator of p53-dependent and -independent apoptosis induced by a wide variety of stimuli. It serves as a proximal signaling molecule whose expression is regulated by transcription factors in response to these stimuli. PUMA transduces death signals primarily to the mitochondria, where it acts indirectly on the Bcl-2 family members Bax and/or Bak by relieving the inhibition imposed by antiapoptotic members. It directly binds and antagonizes all known antiapoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. PUMA ablation or inhibition leads to apoptosis deficiency underlying increased risks for cancer development and therapeutic resistance. It is a general sensor of cell death stimuli and a promising drug target for cancer therapy and tissue damage. It is essential mediator of p53-dependent and p53-independent apoptosis (PMID: 19641508). Catalog #55120-1-AP can recognize PUMA alpha 21-24 kDa and PUMA beta 15-18 kDa.


Product Specific Protocols
WB protocol for PUMA antibody 55120-1-APDownload protocol
IHC protocol for PUMA antibody 55120-1-APDownload protocol
IP protocol for PUMA antibody 55120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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