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  • KD/KO Validated

PUMA Polyclonal antibody

PUMA Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55120-1-AP

Synonyms

BBC3, JFY 1, JFY1, PUMA



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

55120-1-AP targets PUMA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name BCL2 binding component 3
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberNM_014417
Gene symbol BBC3
Gene ID (NCBI) 27113
RRIDAB_10859944
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PUMA, also named as JFY-1 and BBC3, belongs to the Bcl-2 family. It is a critical mediator of p53-dependent and -independent apoptosis induced by a wide variety of stimuli. It serves as a proximal signaling molecule whose expression is regulated by transcription factors in response to these stimuli. PUMA transduces death signals primarily to the mitochondria, where it acts indirectly on the Bcl-2 family members Bax and/or Bak by relieving the inhibition imposed by antiapoptotic members. It directly binds and antagonizes all known antiapoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. PUMA ablation or inhibition leads to apoptosis deficiency underlying increased risks for cancer development and therapeutic resistance. It is a general sensor of cell death stimuli and a promising drug target for cancer therapy and tissue damage. It is essential mediator of p53-dependent and p53-independent apoptosis (PMID: 19641508). Catalog #55120-1-AP can recognize PUMA alpha 21-24 kDa and PUMA beta 15-18 kDa.

Protocols

Product Specific Protocols
WB protocol for PUMA antibody 55120-1-APDownload protocol
IHC protocol for PUMA antibody 55120-1-APDownload protocol
IP protocol for PUMA antibody 55120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanWB

Sci Adv

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Authors - Pengbo Cao
humanWB

Nat Commun

A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.

Authors - Yufeng Yao
mouseWB

J Clin Invest

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Authors - Xue Gong
mouseWB

Cell Death Differ

Mitophagy protein PINK1 suppresses colon tumor growth by metabolic reprogramming via p53 activation and reducing acetyl-CoA production.

Authors - Kunlun Yin
humanWB

Environ Pollut

Insight into the negative impact of ionic liquid: A cytotoxicity mechanism of 1-methyl-3-octylimidazolium bromide.

Authors - Junguo Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah-Eve (Verified Customer) (03-01-2023)

Very good working, pvdf membrane, blocking solution skim milk. One sharp band at the expected size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: Human pulmonary artery smooth muscle cells
PUMA Antibody Western Blot validation (1:2500 dilution) in Human pulmonary artery smooth muscle cells (Cat no:55120-1-AP)