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PUMA Polyclonal antibody

PUMA Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55120-1-AP

Synonyms

BBC3, JFY 1, JFY1, PUMA



Tested Applications

Positive WB detected inA549 cells, mouse heart tissue, HepG2 cells, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:500
Sample-dependent, check data in validation data gallery

Product Information

55120-1-AP targets PUMA in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name BCL2 binding component 3
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberNM_014417
Gene symbol BBC3
Gene ID (NCBI) 27113
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PUMA, also named as JFY-1 and BBC3, belongs to the Bcl-2 family. It is a critical mediator of p53-dependent and -independent apoptosis induced by a wide variety of stimuli. It serves as a proximal signaling molecule whose expression is regulated by transcription factors in response to these stimuli. PUMA transduces death signals primarily to the mitochondria, where it acts indirectly on the Bcl-2 family members Bax and/or Bak by relieving the inhibition imposed by antiapoptotic members. It directly binds and antagonizes all known antiapoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. PUMA ablation or inhibition leads to apoptosis deficiency underlying increased risks for cancer development and therapeutic resistance. It is a general sensor of cell death stimuli and a promising drug target for cancer therapy and tissue damage. It is essential mediator of p53-dependent and p53-independent apoptosis (PMID: 19641508). Catalog #55120-1-AP can recognize PUMA alpha 21-24 kDa and PUMA beta 15-18 kDa.

Protocols

Product Specific Protocols
WB protocol for PUMA antibody 55120-1-APDownload protocol
IHC protocol for PUMA antibody 55120-1-APDownload protocol
IP protocol for PUMA antibody 55120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Target Oncol

Venetoclax Synergistically Enhances the Anti-leukemic Activity of Vosaroxin Against Acute Myeloid Leukemia Cells Ex Vivo.

Authors - Fangbing Liu
humanWB

Front Oncol

The Natural Compound Neobractatin Induces Cell Cycle Arrest by Regulating E2F1 and Gadd45α.

Authors - Zhaoqing Zheng
humanWB

Int J Mol Sci

Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells.

Authors - Yang Gao
humanWB

Cell Death Dis

p53 dynamics orchestrates with binding affinity to target genes for cell fate decision.

Authors - Mengqiu Wu
humanWB

Toxicol Appl Pharmacol

Fluoride induces apoptosis via inhibiting SIRT1 activity to activate mitochondrial p53 pathway in human neuroblastoma SH-SY5Y cells.

Authors - Wei Tu
humanWB

Environ Pollut

Insight into the negative impact of ionic liquid: A cytotoxicity mechanism of 1-methyl-3-octylimidazolium bromide.

Authors - Junguo Ma