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PUMA Polyclonal antibody
PUMA Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IP, IHC, ELISA
Cat no : 55120-1-AP
|Positive WB detected in||mouse heart tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human testis tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 49 publications below|
|IHC||See 3 publications below|
55120-1-AP targets PUMA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Full Name||BCL2 binding component 3|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||18-21 kDa|
|GenBank accession number||NM_014417|
|Gene ID (NCBI)||27113|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PUMA, also named as JFY-1 and BBC3, belongs to the Bcl-2 family. It is a critical mediator of p53-dependent and -independent apoptosis induced by a wide variety of stimuli. It serves as a proximal signaling molecule whose expression is regulated by transcription factors in response to these stimuli. PUMA transduces death signals primarily to the mitochondria, where it acts indirectly on the Bcl-2 family members Bax and/or Bak by relieving the inhibition imposed by antiapoptotic members. It directly binds and antagonizes all known antiapoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. PUMA ablation or inhibition leads to apoptosis deficiency underlying increased risks for cancer development and therapeutic resistance. It is a general sensor of cell death stimuli and a promising drug target for cancer therapy and tissue damage. It is essential mediator of p53-dependent and p53-independent apoptosis (PMID: 19641508). Catalog #55120-1-AP can recognize PUMA alpha 21-24 kDa and PUMA beta 15-18 kDa.
|Product Specific Protocols|
|WB protocol for PUMA antibody 55120-1-AP||Download protocol|
|IHC protocol for PUMA antibody 55120-1-AP||Download protocol|
|IP protocol for PUMA antibody 55120-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.
A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.
J Clin Invest
Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.
Cell Death Differ
Mitophagy protein PINK1 suppresses colon tumor growth by metabolic reprogramming via p53 activation and reducing acetyl-CoA production.
The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah-Eve (Verified Customer) (03-01-2023)
Very good working, pvdf membrane, blocking solution skim milk. One sharp band at the expected size