PUS3 Polyclonal antibody

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  WB analysis of A549 using 17248-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17248-1-AP (PUS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17248-1-AP (PUS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PUS3 Polyclonal Antibody for WB, ELISA

Cat No. 17248-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

2610020J05Rik, FKSG32, pseudouridylate synthase 3, PUS3, tRNA pseudouridine synthase 3, tRNA uridine isomerase 3

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

17248-1-AP targets PUS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PUS3 fusion protein Ag9983
• Full Name: pseudouridylate synthase 3
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 50-52 kDa
• GenBank Accession Number: BC004822
• Gene Symbol: PUS3
• Gene ID (NCBI): 83480
• RRID: AB_2237874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZE2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	iScience; RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma; Authors - Hui Han
human	WB	Anal Chem; Targeted Quantitative Profiling of Epitranscriptomic Reader, Writer, and Eraser Proteins Using Stable Isotope-Labeled Peptides; Authors - Tianyu F Qi