Nectin-1/PVRL1 Polyclonal antibody

Nectin-1/PVRL1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24713-1-AP

Synonyms

CD111, CLPED1, ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, HIgR, HVEC, nectin 1, Nectin-1, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK 12



Tested Applications

Positive WB detected inSH-SY5Y cells, SKOV-3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24713-1-AP is Nectin-1/PVRL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nectin-1/PVRL1 fusion protein Ag20074
Full Name poliovirus receptor-related 1 (herpesvirus entry mediator C)
Calculated molecular weight 517 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC104948
Gene symbol PVRL1
Gene ID (NCBI) 5818
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Nectin-1/PVRL1 antibody 24713-1-APDownload protocol
IHC protocol for Nectin-1/PVRL1 antibody 24713-1-APDownload protocol
IF protocol for Nectin-1/PVRL1 antibody 24713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Mol Ther Oncolytics

Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma.

Authors - Gaku Takano
humanWB

PLoS Pathog

Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1.

Authors - Hiroki Takeuchi