Tested Applications
| Positive WB detected in | mouse brain tissue, SKOV-3 cells, SH-SY5Y cells, mouse cerebellum tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24713-1-AP targets Nectin-1/PVRL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20074 Product name: Recombinant human PVRL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-334 aa of BC104948 Sequence: QVVQVNDSMYGFIGTDVVLHCSFANPLPSVKITQVTWQKSTNGSKQNVAIYNPSMGVSVLAPYRERVEFLRPSFTDGTIRLSRLELEDEGVYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRAKKGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHQQSLACIVNYHMDRFKESLTLNVQYEPEVTIEGFDGNWYLQRMDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFKGPINYSLAGTYICEATNPIGTRSGQVEVNIT Predict reactive species |
| Full Name | poliovirus receptor-related 1 (herpesvirus entry mediator C) |
| Calculated Molecular Weight | 517 aa, 57 kDa |
| Observed Molecular Weight | 90 kDa, 57 kDa |
| GenBank Accession Number | BC104948 |
| Gene Symbol | Nectin-1 |
| Gene ID (NCBI) | 5818 |
| RRID | AB_2879685 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15223 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nectins are a family of immunoglobulin-like cell adhesion molecules comprising four members, nectin-1 to nectin-4, each consisting of an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain (PMID: 12901789, 18703497). Nectins are involved in cell adhesion, migration, polarization, morphogenesis, proliferation, differentiation, and survival. Nectin-1, also known as PVRL1, HVEC and CD111, is widely expressed in a variety of cell types including epithelial cells, neurons, and fibroblasts (PMID: 12901789). It promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Nectin-1 acts as a receptor for several alphaherpesviruses, including herpes simplex viruses (HSV) 1 and 2, porcine pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1) (PMID: 9616127). Nectin-1 is a glycoprotein, the glycosylated form has a larger apparent molecular weight than the calculated molecular weight of 57 kDa (PMID: 12376527; 15731277).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Nectin-1/PVRL1 antibody 24713-1-AP | Download protocol |
| IHC protocol for Nectin-1/PVRL1 antibody 24713-1-AP | Download protocol |
| WB protocol for Nectin-1/PVRL1 antibody 24713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Nectin-1/PVRL1 Polyclonal antibody (Cat# 24713-1-AP) has 11 citations published in journals including Nature, Cell Reports, PLoS Pathogens, Journal of Virology, Frontiers in Oncology, Frontiers in Cellular and Infection Microbiology, Molecular Therapy Oncolytics, BMC Veterinary Research, Heliyon, and bioRxiv. Associated research fields encompass virology (HSV and pseudorabies virus), cell adhesion, oncology (hepatocellular and oral carcinoma), neurology, infectious disease, and obstetrics.
| Species | Application | Title |
|---|---|---|
Nature TMEFF1 is a neuron-specific restriction factor for herpes simplex virus
| ||
Cell Rep Enucleated cells with Nectin-1 overexpression capture HSV-1 and promote viral elimination for herpes simplex encephalitis therapy. | ||
Front Cell Infect Microbiol Aryl hydrocarbon receptor facilitates HSV-1 lytic infection by enhancing viral gene transcription and receptor expression | ||
PLoS Pathog Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1. | ||
Mol Ther Oncolytics Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma. | ||
J Virol Neuropilin-1 Facilitates Pseudorabies Virus Replication and Viral Glycoprotein B Promotes Its Degradation in a Furin-Dependent Manner |
Reviews
What customers say
The single review for 24713-1-AP (PVRL1) reports that the antibody performed well in Western Blot at a 1:1000 dilution. Clear detection was achieved in the ovarian cancer cell line OV8, while detection in OV90 was more difficult, though a similar trend was still apparent. The reviewer rated the product 3 out of 5. Overall, the feedback suggests the reagent works reliably for its intended target in ovarian cancer cell lines, with somewhat variable signal strength depending on the specific cell line tested.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Morgan (Verified Customer) (08-09-2021) | This PVRL1 worked well with the ovarian cancer cell line OV8. It was more difficult to get a clear detection in the ovarian cancer cell line OV90, but we believe a similar trend was apparent.
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