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  • KD/KO Validated

Nectin-1/PVRL1 Polyclonal antibody

Nectin-1/PVRL1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24713-1-AP

Synonyms

CD111, CLPED1, ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, HIgR, HVEC, nectin 1, Nectin-1, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK 12



Tested Applications

Positive WB detected inmouse brain tissue, SKOV-3 cells, SH-SY5Y cells, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

24713-1-AP targets Nectin-1/PVRL1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nectin-1/PVRL1 fusion protein Ag20074
Full Name poliovirus receptor-related 1 (herpesvirus entry mediator C)
Calculated molecular weight 517 aa, 57 kDa
Observed molecular weight90 kDa, 57 kDa
GenBank accession numberBC104948
Gene symbol PVRL1
Gene ID (NCBI) 5818
RRIDAB_2879685
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nectins are a family of immunoglobulin-like cell adhesion molecules comprising four members, nectin-1 to nectin-4, each consisting of an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain (PMID: 12901789, 18703497). Nectins are involved in cell adhesion, migration, polarization, morphogenesis, proliferation, differentiation, and survival. Nectin-1, also known as PVRL1, HVEC and CD111, is widely expressed in a variety of cell types including epithelial cells, neurons, and fibroblasts (PMID: 12901789). It promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Nectin-1 acts as a receptor for several alphaherpesviruses, including herpes simplex viruses (HSV) 1 and 2, porcine pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1) (PMID: 9616127). Nectin-1 is a glycoprotein, the glycosylated form has a larger apparent molecular weight than the calculated molecular weight of 57 kDa (PMID: 12376527; 15731277).

Protocols

Product Specific Protocols
WB protocol for Nectin-1/PVRL1 antibody 24713-1-APDownload protocol
IHC protocol for Nectin-1/PVRL1 antibody 24713-1-APDownload protocol
IF protocol for Nectin-1/PVRL1 antibody 24713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1.

Authors - Hiroki Takeuchi
humanIHC,WB

Front Oncol

High expression of nectin-1 indicates a poor prognosis and promotes metastasis in hepatocellular carcinoma

Authors - Xuequan Wang
  • KD Validated
mouseIHC

Mol Ther Oncolytics

Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma.

Authors - Gaku Takano
humanIHC

Heliyon

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

Authors - Lingyan Chen
pigWB,IF

J Virol

TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication

Authors - Xiu-Qing Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Morgan (Verified Customer) (08-09-2021)

This PVRL1 worked well with the ovarian cancer cell line OV8. It was more difficult to get a clear detection in the ovarian cancer cell line OV90, but we believe a similar trend was apparent.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: OV8 and 0V90
Nectin-1/PVRL1 Antibody Western Blot validation (1:1000 dilution) in OV8 and 0V90 (Cat no:24713-1-AP)