PYROXD2 Polyclonal antibody

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  WB analysis using 21845-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21845-1-AP (PYROXD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 21845-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 21845-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 21845-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 21845-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PYROXD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21845-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C10orf33, FP3420, YueF

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Tested Applications

• Positive WB detected in: A375 cells, HEK-293T cells, L02 cells
• Positive IHC detected in: mouse liver tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21845-1-AP targets PYROXD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PYROXD2 fusion protein Ag15004
• Full Name: chromosome 10 open reading frame 33
• Calculated Molecular Weight: 581 aa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC006131
• Gene Symbol: PYROXD2
• Gene ID (NCBI): 84795
• RRID: AB_3669377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N2H3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pyridine Nucleotide-Disulfide Oxidoreductase Domain 2 (PYROXD2, previously called YueF, C10orf33) is a mitochondrial inner membrane/matrix-residing protein and is reported to regulate mitochondrial function. PYROXD2 is highly expressed in the cytoplasm of normal cells and tissues but is expressed at lower levels in corresponding cancer cells, including liver, lung and renal cell carcinoma and bladder cancer cells (PMID: 29048625, PMID: 35606887).