Phospho-ACC1 (Ser79) Polyclonal antibody

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• Phospho-ACC1 (Ser79) Polyclonal antibody (29119-1-AP)

Phospho-ACC1 (Ser79) Polyclonal Antibody for WB, IF-P, FC (Intra), ELISA

Cat No. 29119-1-AP Copied!

Publications (57)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Synonyms

ACC1 (Ser79), ACC1 Ser79, Acetyl Coenzyme A Carboxylase (phospho S79), p ACC1 (Ser79), p-ACC1

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  WB analysis using 29119-1-AP

  Non-treated SH-SY5Y, phosphatase inhibitor treated and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 29119-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 29119-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Phospho-ACC1 (Ser79) antibody (29119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 29119-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Phospho-ACC1 (Ser79) antibody (29119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of NIH/3T3 using 29119-1-AP

  1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-ACC1 (Ser79) Polyclonal antibody (29119-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, NIH/3T3 cells
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29119-1-AP targets Phospho-ACC1 (Ser79) in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: acetyl-Coenzyme A carboxylase alpha
• Calculated Molecular Weight: 2383 aa, 275 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC137287
• Gene Symbol: Acetyl-CoA Carboxylase 1
• Gene ID (NCBI): 31
• RRID: AB_2918238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13085
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)

Publications

What published studies show

The Phospho-ACC1 (Ser79) Polyclonal antibody (Cat# 29119-1-AP) has 57 citations. Key journals include Signal Transduction and Targeted Therapy, Autophagy, Science Advances, Journal of Advanced Research, Acta Pharmaceutica Sinica B, Cell Reports, Journal of Ethnopharmacology, Food Function, Nutrients, and FASEB Journal. Research fields span lipid metabolism, non-alcoholic fatty liver disease (NAFLD/MASLD), obesity, AMPK/ACC signaling, ferroptosis, autophagy, and cancer (hepatocellular, lung, ovarian, colorectal), with most studies using Western blot in human, mouse, and rat models.

Automatically generated from the 57 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease; Authors - Chandana Thimme Gowda
human	WB	Autophagy; PRKAB2 as a tumor suppressor in renal cell carcinoma: inhibiting mitophagy via the LRPPRC-PRKN/parkin interaction and cardiolipin biosynthesis.; Authors - Kailei Chen
human	WB	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	WB	J Adv Res; Sodium citrate targeting Ca2+/CAMKK2 pathway exhibits anti-tumor activity through inducing apoptosis and ferroptosis in ovarian cancer; Authors - Yulun Wu
mouse,human	WB	Acta Pharm Sin B; Schisanhenol ameliorates non-alcoholic fatty liver disease via inhibiting miR-802 activation of AMPK-mediated modulation of hepatic lipid metabolism; Authors - Bin Li
mouse	WB	Sci Adv; Liver endothelial zonation orchestrates hepatic steatosis onset through retinoic acid-regulated FGF1.; Authors - Zhiqiang Fang