Phospho-ACC1 (Ser79) Polyclonal antibody

Phospho-ACC1 (Ser79) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29119-1-AP

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1, Phospho-ACC (Ser79)



Tested Applications

Positive WB detected inλ phosphatase treated SH-SY5Y cells, λ phosphatase treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29119-1-AP targets Phospho-ACC1 (Ser79) in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name acetyl-Coenzyme A carboxylase alpha
Calculated Molecular Weight 2383 aa, 275 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC137287
Gene Symbol Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
RRIDAB_2918238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)

Protocols

Product Specific Protocols
WB protocol for Phospho-ACC1 (Ser79) antibody 29119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
mouseWB,IHC

Food Funct

Hawk tea prevents high-fat diet-induced obesity in mice by activating the AMPK/ACC/SREBP1c signaling pathways and regulating the gut microbiota.

Authors - Wei Tao
mouseWB

Front Cardiovasc Med

MCC950, a Selective NLRP3 Inhibitor, Attenuates Adverse Cardiac Remodeling Following Heart Failure Through Improving the Cardiometabolic Dysfunction in Obese Mice.

Authors - Menglong Wang
humanWB

J Nat Prod

Hernandezine Regulates Proliferation and Autophagy-Induced Apoptosis in Melanoma Cells.

Authors - Xiaoting Wang
ratWB

Nutrients

Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats

Authors - Yujie Zhong
mouseWB

J Agric Food Chem

Modulation of the Microbiome-Fat-Liver Axis by Lactic Acid Bacteria: A Potential Alleviated Role in High-Fat-Diet-Induced Obese Mice

Authors - Benliang Wei