Phospho-AKT (Thr308) Polyclonal antibody

Phospho-AKT (Thr308) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


Human and More (2)





Cat no : 29163-1-AP


AKT, AKT1, Phospho-AKT (Thr308), PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

Tested Applications

Positive WB detected inCalyculin A treated HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Sample-dependent, check data in validation data gallery

Product Information

29163-1-AP targets Phospho-AKT (Thr308) in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight58 kDa
GenBank accession numberBC000479
Gene symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672)


Product Specific Protocols
WB protocol for Phospho-AKT (Thr308) antibody 29163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Pharmacol

Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells.

Authors - Ming Xin


Circadian Period 2 (Per2) downregulate inhibitor of differentiation 3 (Id3) expression via PTEN/AKT/Smad5 axis to inhibits glioma cell proliferation.

Authors - Yifan Zhang

Physiol Res

6-Hydroxydopamine-Induced Neurotoxicity in Rat Model of Parkinson's Disease: Is Reversed via Anti-Oxidative Activities of Curcumin and Aerobic Exercise Therapy

Authors - H Sokouti

Biotechnol Lett

BMSCs improve TNBS-induced colitis in rats by inducing Treg differentiation by expressing PD-L1

Authors - Fei Gao

Cell Biol Int

RNA-seq and ATAC-seq analyses of multilineage differentiating stress enduring cells: Comparison with dermal fibroblasts

Authors - Xiao-Qian Mao

Cell Death Dis

Fatostatin induces ferroptosis through inhibition of the AKT/mTORC1/GPX4 signaling pathway in glioblastoma

Authors - Jiayang Cai