Phospho-ASK1 (Ser966) Polyclonal antibody

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  WB analysis using 28846-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28846-1-AP (Phospho-ASK1 (Ser966) antibody) at dilution of 1:500 incubated at 4℃ overnight.

Phospho-ASK1 (Ser966) Polyclonal Antibody for WB, ELISA

Cat No. 28846-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (3)

Applications

WB, ELISA

Synonyms

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Phospho-ASK1 (Ser966)

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Tested Applications

• Positive WB detected in: λ phosphatase treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below

Product Information

28846-1-AP targets Phospho-ASK1 (Ser966) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase kinase 5
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC054503
• Gene Symbol: ASK1
• Gene ID (NCBI): 4217
• RRID: AB_2881220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99683
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. The predicted MW of ASK1 is around 155 kDa and the 110-120 kDa fragment has also been reported.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Saudi Pharm J; iTRAQ-derived quantitative proteomics uncovers the neuroprotective property of bexarotene in a mice model of cerebral ischemia-reperfusion injury.; Authors - Hailin Liu
mouse	WB	Cell Death Discov; Dual function of peroxiredoxin I in lipopolysaccharide-induced osteoblast apoptosis via reactive oxygen species and the apoptosis signal-regulating kinase 1 signaling pathway.; Authors - Hao Feng
	WB	Virol J; HPV16 E7 inhibits HBD2 expression by down-regulation of ASK1-p38 MAPK pathway in cervical cancer; Authors - Juanjuan Liao
human,mouse	WB	J Ethnopharmacol; Ginsenoside Rg1 attenuates T2DM-induced renal damage and fibrosis by inhibiting TRPC6-ChREBP-TXNIP signaling; Authors - Hui Zhang
human	WB	Oncol Rep; ABT‑737 increases cisplatin sensitivity through the ROS‑ASK1‑JNK MAPK signaling axis in human ovarian cancer cisplatin‑resistant A2780/DDP cells; Authors - Xiaoning Li
rat	WB	Int Immunopharmacol; Promotion of Raf-1/ASK1 complex formation by corylin inhibits cell apoptosis in myocardial ischemia/reperfusion injury; Authors - Kaiyu Huang