Phospho-Chk1 (Ser296) Polyclonal antibody

Phospho-Chk1 (Ser296) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat and More (1)





Cat no : 28805-1-AP



Tested Applications

Positive WB detected inCalyculin A treated HEK-293T cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

28805-1-AP targets Phospho-Chk1 (Ser296) in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated Molecular Weight 54 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC004202
Gene Symbol CHEK1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. CHK1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. CHK1 can be autophosphorylated (PMID:22941630) and ubiquitinated (PMID:19276361). Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. Activation occurs in response to blocked DNA replication and certain forms of genotoxic stress.


Product Specific Protocols
WB protocol for Phospho-Chk1 (Ser296) antibody 28805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Arch Toxicol

SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice

Authors - Jia-Wen Chen

Cell Prolif

eIF3a-PPP2R5A-mediated ATM/ATR dephosphorylation is essential for irinotecan-induced DNA damage response.

Authors - Chao Mei

Adv Sci (Weinh)

Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis

Authors - Hyeoncheol Kim

Research (Wash D C)

Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer

Authors - Yangyang Li