Phospho-Caspase 9 (Ser196) Polyclonal antibody

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  WB analysis using 28794-1-AP

  Non-treated HEK-293T and calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28794-1-AP (Phospho-Caspase 9 (Ser196) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-Caspase 9 (Ser196) Polyclonal Antibody for WB, ELISA

Cat No. 28794-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, CASPASE 9c, Caspase9, ICE LAP6, ICE like apoptotic protease 6, MCH6

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

28794-1-AP targets Phospho-Caspase 9 (Ser196) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 9, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC002452
• Gene Symbol: Caspase 9
• Gene ID (NCBI): 842
• RRID: AB_2881214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55211
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46 kDa precursor protein which can be cleaved into a 35 kDa subunit and a 11 kDa subunit. Control of all caspases is tightly regulated by a series of phosphorylation events enacted by several different kinases. Caspase-9 is the most heavily phosphorylated of all caspases, with phosphorylation of at least 11 distinct residues in all three caspase-9 domains by nine kinases. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507, PMID: 29066624)

Publications

Species	Application	Title
human	WB	Front Immunol; Salmonella Infantis Delays the Death of Infected Epithelial Cells to Aggravate Bacterial Load by Intermittent Phosphorylation of Akt With SopB.; Authors - Bing-Xin Chu
human	WB	Sci Adv; Stromal-derived MAOB promotes prostate cancer growth and progression; Authors - Tianjie Pu