Phospho-Caspase 9 (Ser196) Polyclonal antibody

Phospho-Caspase 9 (Ser196) Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB

Conjugate

Unconjugated

Publications

1

Cat no : 28794-1-AP

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, CASPASE 9c, Caspase9, ICE LAP6, ICE like apoptotic protease 6, MCH6, Phospho-Caspase 9 (Ser196)

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Validation Data Gallery

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  WB analysis using 28794-1-AP

  Non-treated HEK-293T and calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28794-1-AP (Phospho-Caspase 9 (Ser196) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

28794-1-AP targets Phospho-Caspase 9 (Ser196) in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 9, apoptosis-related cysteine peptidase
• Calculated molecular weight: 46 kDa
• Observed molecular weight: 36 kDa
• GenBank accession number: BC002452
• Gene symbol: CASP9
• Gene ID (NCBI): 842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Front Immunol; Salmonella Infantis Delays the Death of Infected Epithelial Cells to Aggravate Bacterial Load by Intermittent Phosphorylation of Akt With SopB.; Authors - Bing-Xin Chu