Phospho-EGFR (Tyr1069) Polyclonal antibody

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• Phospho-EGFR (Tyr1069) Polyclonal antibody (30277-1-AP)

Phospho-EGFR (Tyr1069) Polyclonal Antibody for WB, ELISA

Cat No. 30277-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IF, ELISA

Synonyms

EGFR (Tyr1069), p EGFR , p EGFR (Tyr1069), p EGFR Tyr1069, p-EGFR

• 

  WB analysis using 30277-1-AP

  Non-treated HeLa and EGF (HZ-1326) treated HeLa cells were subjected to SDS PAGE followed by western blot with 30277-1-AP (Phospho-EGFR (Tyr1069) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody (66305-1-Ig) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Schematic diagram of the phosphorylation sites of EGFR.

  In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: EGF treated HeLa cells

This antibody, also known as EGFR Tyr1045, is the site where the signal peptide is removed.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30277-1-AP targets Phospho-EGFR (Tyr1069) in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 1210 aa, 134 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC094761
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• RRID: AB_3085349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00533
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Phosphorylation of EGFR Tyr1069 acts as the degron of EGFR and is recognized by c-Cbl, enhancing the ubiquitination and degradation of EGFR. EGFR stability is tightly regulated upon EGF stimulation. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1069 as EGFR Tyr1045, the site where the signaling peptide is removed.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
bovine	WB	Theriogenology; LRRC46 regulates bovine granulosa cell functions via the COL8A1-mediated EGFR/PI3K/AKT signaling pathway.; Authors - Bowen Cheng
human	WB	J Autoimmun; Mechanistic studies on the regulation of systemic sclerosis progression by imbalance in angiogenic T cell subsets.; Authors - Haomiao Shen
human	WB	J Mol Histol; Study on the mechanism of costunolide inhibiting triple-negative breast cancer by EGFR ubiquitination and degradation to suppress the ERK/AKT signaling pathway.; Authors - Ying Li
mouse	WB	Biomaterials; Engineered bacterial outer membrane vesicles enhanced tumor immunotherapy through remodeling tumor stroma and targeted delivery of CD73 siRNA.; Authors - Lili Cheng
human	WB	Cytojournal; Investigating the anti-cancer potential of sulfatase 1 and its underlying mechanism in non-small cell lung cancer; Authors - Bingling Zhang
human	WB	Naunyn Schmiedebergs Arch Pharmacol; Integrated experimental and network pharmacology analyses reveal inhibitory effects of albiflorin on renal cell carcinoma cells.; Authors - Kai Meng