|Positive WB detected in||Calyculin A treated PC-3 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Sample-dependent, check data in validation data gallery|
28740-1-AP targets Phospho-EIF2S1 (Ser51) in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||NM_004094|
|Gene ID (NCBI)||1965|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51
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