Tested Applications
| Positive WB detected in | PC-3 cells, λ phosphatase treated PC-3 cells |
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28779-1-AP targets Phospho-EIF4B (Ser406) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | eukaryotic translation initiation factor 4B |
| Calculated Molecular Weight | 611 aa, 69 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC073154 |
| Gene Symbol | EIF4B |
| Gene ID (NCBI) | 1975 |
| RRID | AB_2918201 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23588 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623). EIF4B Ser406 was identified as a novel phosphorylation site regulated by mitogens, and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-EIF4B (Ser406) antibody 28779-1-AP | Download protocol |
| WB protocol for Phospho-EIF4B (Ser406) antibody 28779-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-EIF4B (Ser406) Polyclonal antibody (Cat# 28779-1-AP) has 1 citation to date. It appears in Acta Pharmacologica Sinica, in a study investigating how gambogenic acid regulates translational processes in Nrf2-mutant esophageal squamous cell carcinoma through Nrf2/MafG protein multimerization. The associated research field encompasses translational regulation, the Nrf2 signaling pathway, and esophageal squamous cell carcinoma biology.
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Gambogenic acid globally regulates translational processes in Nrf2-mutant esophageal squamous cell carcinoma by promoting Nrf2/MafG protein multimerization. |
