Phospho-EIF4B (Ser406) Polyclonal antibody

Phospho-EIF4B (Ser406) Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 28779-1-AP


EIF 4B, EIF4B, Phospho-EIF4B (Ser406), PRO1843

Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells
Positive IF detected insodium arsenite treated HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28779-1-AP targets Phospho-EIF4B (Ser406) in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name eukaryotic translation initiation factor 4B
Calculated Molecular Weight 611 aa, 69 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC073154
Gene Symbol EIF4B
Gene ID (NCBI) 1975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623). EIF4B Ser406 was identified as a novel phosphorylation site regulated by mitogens, and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.


Product Specific Protocols
WB protocol for Phospho-EIF4B (Ser406) antibody 28779-1-APDownload protocol
IF protocol for Phospho-EIF4B (Ser406) antibody 28779-1-APDownload protocol
Standard Protocols
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