Phospho-EIF4B (Ser406) Recombinant antibody

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  WB analysis using 80059-1-RR

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 80059-1-RR

  WB result of Phospho-EIF4B (Ser406) antibody (80059-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-EIF4B (Ser406) transfected HeLa cells.

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  IHC staining of Jurkat using 80059-1-RR

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Phospho-EIF4B (Ser406) Recombinant Antibody for WB, IHC, ELISA

Cat No. 80059-1-RR

Clone No.5G14

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EIF4B, PRO1843, eIF-4B, EIF 4B, 5G14

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Calyculin A treated HEK-293T cells, PC-3 cells, Calyculin A treated PC-3 cells, Calyculin A treated Hela cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: Jurkat cells; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

80059-1-RR targets Phospho-EIF4B (Ser406) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 4B
• Calculated Molecular Weight: 611 aa, 69 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC073154
• Gene Symbol: EIF4B
• Gene ID (NCBI): 1975
• RRID: AB_2918859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23588
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; Lactate-upregulated ARG2 expression induces cellular senescence in fibroblast-like synoviocytes of osteoarthritis via activating the mTOR/S6K1 signaling pathway; Authors - Yifan Huang