Phospho-EIF4B (Ser406) Recombinant antibody
Phospho-EIF4B (Ser406) Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB
Conjugate
Unconjugated
CloneNo.
5G14
Cat no : 80059-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, Calyculin A treated HEK-293T cells, PC-3 cells, Calyculin A treated PC-3 cells, Hela cells, Calyculin A treated Hela cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Sample-dependent, check data in validation data gallery |
Product Information
80059-1-RR targets Phospho-EIF4B (Ser406) in WB applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | eukaryotic translation initiation factor 4B |
Calculated molecular weight | 611 aa, 69 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC073154 |
Gene symbol | EIF4B |
Gene ID (NCBI) | 1975 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-EIF4B (Ser406) antibody 80059-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |