Phospho-FOXO3A (Ser315) Polyclonal antibody

Phospho-FOXO3A (Ser315) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat and More (1)





Cat no : 28755-1-AP


AF6q21, AF6q21 protein, DKFZp781A0677, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A, Phospho-FOXO3A (Ser315), S315, Ser315

Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28755-1-AP targets Phospho-FOXO3A (Ser315) in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name forkhead box O3
Calculated Molecular Weight 673 aa, 71 kDa
Observed Molecular Weight80-90 kDa
GenBank Accession NumberBC021224
Gene Symbol FOXO3
Gene ID (NCBI) 2309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa full length FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386). IKBKE directly activates Akt and that Akt inhibits FOXO3a by phosphorylation of Ser32, Ser253 and Ser315.


Product Specific Protocols
WB protocol for Phospho-FOXO3A (Ser315) antibody 28755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Transl Med

METTL3-mediated m6A modification of SIRT1 mRNA inhibits progression of endometriosis by cellular senescence enhancing

Authors - Xiaotong Wang

Exp Cell Res

Alleviation of CCCP-induced mitochondrial injury by augmenter of liver regeneration via the PINK1/Parkin pathway-dependent mitophagy.

Authors - Jing Zhang

Front Biosci (Landmark Ed)

The Investigation of the Molecular Mechanism of Morinda officinalis How in the Treatment of Heart Failure

Authors - Aiping Wang

Phytother Res

Berberine ameliorates contrast-induced acute kidney injury by regulating HDAC4-FoxO3a axis-induced autophagy: In vivo and in vitro

Authors - Zhi Zuo

J Ethnopharmacol

Maimendong decoction regulates M2 macrophage polarization to suppress pulmonary fibrosis via PI3K/Akt/FOXO3a signalling pathway-mediated fibroblast activation

Authors - He Shuangshuang

Int Immunopharmacol

The metabolite butyrate produced by gut microbiota inhibits cachexia-associated skeletal muscle atrophy by regulating intestinal barrier function and macrophage polarization

Authors - Hao Liu