Phospho-FOXO3A (Ser315) Polyclonal antibody

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  WB analysis using 28755-1-AP

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28755-1-AP (Phospho-FOXO3A (Ser315) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 28755-1-AP

  Non-treated and TGF beta 1 treated HeLa cells were subjected to SDS PAGE followed by western blot with 28755-1-AP (Phospho-FOXO3A (Ser315) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28755-1-AP

  Non treated and Forskolin treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28755-1-AP (Phospho-FOXO3A (Ser315) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Phospho-FOXO3A (Ser315) Polyclonal Antibody for WB, ELISA

Cat No. 28755-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

FOXO3, FOXO3A, Forkhead box protein O3, forkhead box O3, FKHRL1P2

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Tested Applications

• Positive WB detected in: Calyculin A treated PC-3 cells, HeLa cells, PC-3 cells, TGF beta 1 treated HeLa cells, Forskolin treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below

Product Information

28755-1-AP targets Phospho-FOXO3A (Ser315) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: forkhead box O3
• Calculated Molecular Weight: 673 aa, 71 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC021224
• Gene Symbol: FOXO3A
• Gene ID (NCBI): 2309
• RRID: AB_2881210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43524
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa full length FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386). IKBKE directly activates Akt and that Akt inhibits FOXO3a by phosphorylation of Ser32, Ser253 and Ser315.

Publications

Species	Application	Title
human	WB	J Transl Med; METTL3-mediated m6A modification of SIRT1 mRNA inhibits progression of endometriosis by cellular senescence enhancing; Authors - Xiaotong Wang
		Exp Cell Res; Alleviation of CCCP-induced mitochondrial injury by augmenter of liver regeneration via the PINK1/Parkin pathway-dependent mitophagy.; Authors - Jing Zhang
rat	WB	Front Biosci (Landmark Ed); The Investigation of the Molecular Mechanism of Morinda officinalis How in the Treatment of Heart Failure; Authors - Aiping Wang
human,rat	WB	Phytother Res; Berberine ameliorates contrast-induced acute kidney injury by regulating HDAC4-FoxO3a axis-induced autophagy: In vivo and in vitro; Authors - Zhi Zuo
human,mouse	WB	J Ethnopharmacol; Maimendong decoction regulates M2 macrophage polarization to suppress pulmonary fibrosis via PI3K/Akt/FOXO3a signalling pathway-mediated fibroblast activation; Authors - He Shuangshuang
mouse	WB	Int Immunopharmacol; The metabolite butyrate produced by gut microbiota inhibits cachexia-associated skeletal muscle atrophy by regulating intestinal barrier function and macrophage polarization; Authors - Hao Liu