Phospho-GSK3A (Ser21) Polyclonal antibody

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  WB analysis using 28875-1-AP

  Non-treated PC-3 and Calyculin A treated treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28875-1-AP (Phospho-GSK3A (Ser21) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-GSK3A (Ser21) Polyclonal Antibody for WB, ELISA

Cat No. 28875-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

DKFZp686D0638, GSK 3 alpha, GSK3A, Phospho-GSK3A (Ser21)

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Tested Applications

• Positive WB detected in: Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28875-1-AP targets Phospho-GSK3A (Ser21) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glycogen synthase kinase 3 alpha
• Calculated Molecular Weight: 483 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC027984
• Gene Symbol: GSK3A
• Gene ID (NCBI): 2931
• RRID: AB_2918211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49840
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSK3A (glycogen synthase kinase 3 alpha), one of the two functional isoforms encoded by the GSK3 gene, is a serine/threonine protein kinase that is involved in cell-cycle progression, differentiation, and apoptosis (PMID: 27049759). Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin.GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3A and Ser9 of GSK-3B. GSK3A acts as a tumor suppressor and has important roles in acute myeloid leukemia (AML), prostate cancer, and pancreatic cancer (PMID: 22326953, 22539113, 23547054).