Phospho-GSK3A (Ser21) Polyclonal antibody

Phospho-GSK3A (Ser21) Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 28875-1-AP

Synonyms

DKFZp686D0638, GSK 3 alpha, GSK3A, Phospho-GSK3A (Ser21)



Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

28875-1-AP targets Phospho-GSK3A (Ser21) in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 alpha
Calculated molecular weight 483 aa, 51 kDa
Observed molecular weight51 kDa
GenBank accession numberBC027984
Gene symbol GSK3A
Gene ID (NCBI) 2931
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSK3A (glycogen synthase kinase 3 alpha), one of the two functional isoforms encoded by the GSK3 gene, is a serine/threonine protein kinase that is involved in cell-cycle progression, differentiation, and apoptosis (PMID: 27049759). Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin.GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3A and Ser9 of GSK-3B. GSK3A acts as a tumor suppressor and has important roles in acute myeloid leukemia (AML), prostate cancer, and pancreatic cancer (PMID: 22326953, 22539113, 23547054).

Protocols

Product Specific Protocols
WB protocol for Phospho-GSK3A (Ser21) antibody 28875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols