|Positive WB detected in
|λ phosphatase treated HEK-293T cells
|Western Blot (WB)
|WB : 1:500-1:1000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
29125-1-AP targets Phospho-GSK3B (Tyr216) in WB, ELISA applications and shows reactivity with Human samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|glycogen synthase kinase 3 beta
|Calculated molecular weight
|433 aa, 48 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. PI3K pathway downstream of receptor tyrosine kinase signalling is thought to result in inactivation of GSK3 through phosphorylation of the amino-terminal serine residue (Ser9 in GSK3 beta and Ser21 in GSK3 alpha). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704)
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