Tested Applications
| Positive WB detected in | PC-3 cells, Calyculin A treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29125-1-AP targets Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | glycogen synthase kinase 3 beta |
| Calculated Molecular Weight | 433 aa, 48 kDa |
| Observed Molecular Weight | 48-50 kDa |
| GenBank Accession Number | BC000251 |
| Gene Symbol | GSK3B |
| Gene ID (NCBI) | 2932 |
| RRID | AB_2918240 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49841 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. GSK3 beta is activated by phosphorylation of the tyrosine 216 residue (Tyr216) located in the kinase domain and inactivated by phosphorylation of the amino-terminal serine 9 residue (Ser9). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704,PMID: 24722055)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) antibody 29125-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSK3β antibody (Cat# 29125-1-AP) has 27 citations published across 26 journals, including Nature Communications, Advanced Science, Molecular Psychiatry, Journal of Controlled Release, and Cell Signaling. Associated research fields span neuroscience (Alzheimer's disease, Parkinson's disease, neuroinflammation), oncology (breast, gastric, glioma, lung, and colorectal cancers), metabolic disorders (diabetes), fibrosis, osteoporosis, cardiovascular biology, and pharmacology. Western blot is the predominant application, with studies conducted in human, mouse, rat, and rabbit models.
| Species | Application | Title |
|---|---|---|
Nat Commun Discovery of a CNS active GSK3 degrader using orthogonally reactive linker screening. | ||
Adv Sci (Weinh) In Vivo CRISPR Screening Identifies the Glutamate Receptor GRIA2 as Promoting Peritoneal Metastasis of Gastric Cancer via Calcium-Dependent β-Catenin Activation. | ||
J Control Release Bioengineered bacterial extracellular vesicles for targeted delivery of an osteoclastogenesis-inhibitory peptide to alleviate osteoporosis | ||
Phytomedicine The chemosensitizing activity of betulinic acid in suppressing macrophage polarization through GSK-3β/β-catenin/CXCL1 signaling in breast cancer. | ||
Mol Psychiatry PIAS1-mediated GSK3β SUMOylation exacerbates tauopathy and cognitive deficits in Alzheimer's disease models. | ||
Brain Behav Immun Astrocyte-neuron communication through the complement C3-C3aR pathway in Parkinson's disease |
Reviews
What customers say
Both reviews are from the same user (Patricia Hixson) who used this antibody for Western Blot with lot 00093856. She rated it 4/5 in June 2025, saying "Works nicely with my WB," and 5/5 in January 2025, stating "Great Ab. Gave clean results." Overall, the feedback is positive, highlighting reliable performance and clean WB outcomes.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Patricia (Verified Customer) (06-25-2025) | Works nicely with my WB.
|
Patricia (Verified Customer) (01-31-2025) | Great Ab. Gave clean results.
|
