Phospho-GSK3B (Tyr216) Polyclonal antibody

Phospho-GSK3B (Tyr216) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29125-1-AP

Synonyms

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta, Phospho-GSK3B (Tyr216)



Tested Applications

Positive WB detected inλ phosphatase treated HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

29125-1-AP targets Phospho-GSK3B (Tyr216) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 433 aa, 48 kDa
Observed molecular weight50 kDa
GenBank accession numberBC000251
Gene symbol GSK3B
Gene ID (NCBI) 2932
RRIDAB_2918240
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. PI3K pathway downstream of receptor tyrosine kinase signalling is thought to result in inactivation of GSK3 through phosphorylation of the amino-terminal serine residue (Ser9 in GSK3 beta and Ser21 in GSK3 alpha). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704)

Protocols

Product Specific Protocols
WB protocol for Phospho-GSK3B (Tyr216) antibody 29125-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neurochem Res

Involvement of PI3K/AKT Pathway in the Rapid Antidepressant Effects of Crocetin in Mice with Depression-Like Phenotypes

Authors - Susu Lin
ratWB

Evid Based Complement Alternat Med

Sufentanil Alleviates Sepsis-Induced Myocardial Injury and Stress Response in Rats through the ERK/GSK-3β Signaling Axis.

Authors - Hongcheng Zang
humanWB

Mar Drugs

Antidiabetic Effect of Collagen Peptides from Harpadon nehereus Bones in Streptozotocin-Induced Diabetes Mice by Regulating Oxidative Stress and Glucose Metabolism

Authors - Qianxia Lin
mouseWB

Front Aging Neurosci

The γ-Adducin 1-357 fragment promotes tau pathology

Authors - Honglu Yu
mouseWB

Aging (Albany NY)

The Wnt/β-catenin signaling pathway inhibits osteoporosis by regulating the expression of TERT: an in vivo and in vitro study

Authors - Yuanqing Cai