Phospho-Histone H2A.X (Ser139) Polyclonal antibody

Phospho-Histone H2A.X (Ser139) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29380-1-AP

Synonyms

H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x, Phospho-Histone H2A.X Ser139, γH2AX



Tested Applications

Positive WB detected inUV treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29380-1-AP targets Phospho-Histone H2A.X (Ser139) in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name H2A histone family, member X
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC013416
Gene Symbol H2AFX
Gene ID (NCBI) 3014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)

Protocols

Product Specific Protocols
WB protocol for Phospho-Histone H2A.X (Ser139) antibody 29380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Nano

Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy

Authors - Qiqi Zhang
mouseWB

J Nanobiotechnology

Synergistic cerium oxide nanozymes: targeting DNA damage and alleviating tumor hypoxia for improved NSCLC radiotherapy efficiency

Authors - Jie Liu
humanWB

Bioorg Chem

Dual inhibition of oxidative phosphorylation and glycolysis to enhance cancer therapy

Authors - Xi Sheng
mouseIF

Ecotoxicol Environ Saf

2-bromoacetamide exposure impairs oocyte maturation in mice and humans primarily via disrupting the cytoskeleton

Authors - Chuanxin Zhang
humanWB

BMC Pharmacol Toxicol

Panobinostat (LBH589) combined with AM1241 induces cervical cancer cell apoptosis through autophagy pathway

Authors - Bo Sheng
humanWB

Aging (Albany NY)

A prognostic risk model for programmed cell death and revealing TRIB3 as a promising apoptosis suppressor in renal cell carcinoma

Authors - Qingfeng Fu