Tested Applications
| Positive WB detected in | Staurosporine treated Jurkat cells, C6 cells |
| Positive IHC detected in | Jurkat cells, mouse colon tissue, mouse liver tissue, mouse spleen tissue, mouse stomach tissue, rat spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | UV treated HeLa cells |
| Positive FC (Intra) detected in | Staurosporine treated Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83307-2-RR targets Phospho-Histone H2A.X (Ser139) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | H2A histone family, member X |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC013416 |
| Gene Symbol | Histone H2A.X |
| Gene ID (NCBI) | 3014 |
| RRID | AB_3670974 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16104 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RR | Download protocol |
| IHC protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RR | Download protocol |
| WB protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-Histone H2A.X (Ser139) Recombinant monoclonal antibody (83307-2-RR) has 75 citations published in journals including Nature Communications, ACS Nano, Advanced Science, Journal of Medicinal Chemistry, Cell Reports, Journal of Biological Chemistry, FASEB Journal, Oncogene, and Science Advances. Research fields span DNA damage/repair, oncology, aging/senescence, oxidative stress, immunotherapy, cell death (ferroptosis, pyroptosis, necroptosis), drug resistance, and environmental toxicology.
| Species | Application | Title |
|---|---|---|
Imeta Microbial keystone taxa and metabolic signatures in centenarians regulate intestinal homeostasis during aging. | ||
Bioact Mater Reconstructing protein conformations to activate follicular enzyme-nutrient reservoirs for reversing follicle aging. | ||
Drug Resist Updat Host-directed novel mechanistic insights of doxorubicin reveal its efficacy against drug-resistant HSV-1 underscoring risks with oncolytic virotherapy. | ||
Drug Resist Updat Lactate-driven H3K18 lactylation promotes cisplatin resistance in bladder cancer via HNRNPF-Parkin mediated mitophagy. | ||
Drug Resist Updat RAD1 promotes oxaliplatin resistance in gastric cancer by reinforcing NRF2-driven antioxidant defense and DDR checkpoint signaling. | ||
Nat Commun CRL4(DCAF12) regulation of MCMBP ensures optimal licensing of DNA replication. |
Reviews
What customers say
The single review for this H2AX antibody (used in immunofluorescence on A549 cells at 1:400 dilution) rates it 3 out of 5 stars. The reviewer reported low specificity, noting pronounced non-nuclear staining and substantial background signal in control conditions (no irradiation, no IONPs) where no DNA damage should be present. Overall, the feedback highlights concerns about the antibody's ability to discriminate specific nuclear DNA damage signals from non-specific binding.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Eglantine (Verified Customer) (02-10-2026) | The H2AX antibody demonstrates low specificity with pronounced non-nuclear staining. There is also a lot of signal in control conditions (no irradiation, no IONPs) where there should not be any DNA damage.
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