Phospho-JNK (Tyr185) Recombinant antibody

Phospho-JNK (Tyr185) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G8

Cat no : 80024-1-RR

Synonyms

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, Phospho-JNK (Tyr185), PRKM8, SAPK1



Tested Applications

Positive WB detected inHEK-293T cells, UV treated NIH/3T3 cells, UV treated HEK-293T cells, Anisomycin treated NIH/3T3 cells, Calyculin A treated HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 81 publications below

Product Information

80024-1-RR targets Phospho-JNK (Tyr185) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase 8
Observed molecular weight37-45kDa
GenBank accession numberNM_139049
Gene symbol MAPK8
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun.

Protocols

Product Specific Protocols
WB protocol for Phospho-JNK (Tyr185) antibody 80024-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response

Authors - Zhe Wang
humanWB

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
mouseWB

J Hazard Mater

Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders.

Authors - Tianxin Zhao
mouseWB

J Transl Med

Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways

Authors - Xin-Sen Chen
mouseWB

Life Sci

The GSTP1/MAPKs/BIM/SMAC modulatory actions of nitazoxanide: Bioinformatics and experimental evidence in subcutaneous solid Ehrlich carcinoma-inoculated mice

Authors - Samar Imbaby
mouse,ratWB

Pharmacol Res

Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.

Authors - Jiale Cai