Phospho-JNK (Tyr185) Recombinant antibody

Phospho-JNK (Tyr185) Recombinant Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2G8

Cat no : 80024-1-RR

Synonyms

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, Phospho-JNK (Tyr185), PRKM8, SAPK1



Tested Applications

Positive WB detected inHEK-293T cells, UV treated NIH/3T3 cells, UV treated HEK-293T cells, Anisomycin treated NIH/3T3 cells, Calyculin A treated HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 8 publications below

Product Information

80024-1-RR targets Phospho-JNK (Tyr185) in WB,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase 8
Observed molecular weight37-45kDa
GenBank accession numberNM_139049
Gene symbol MAPK8
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun.

Protocols

Product Specific Protocols
WB protocol for Phospho-JNK (Tyr185) antibody 80024-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer.

Authors - Xiaohui Zhang
mouseWB

Dig Dis Sci

Adiponectin Alleviates Intestinal Fibrosis by Enhancing AMP-Activated Protein Kinase Phosphorylation.

Authors - Minghao Xie
humanWB

Arch Pharm Res

Anti-thrombotic activity of phenolic acids obtained from Salvia miltiorrhiza f. alba in TNF-α-stimulated endothelial cells via the NF-κB/JNK/p38 MAPK signaling pathway.

Authors - Xianjing Zheng
humanWB

J Biochem Mol Toxicol

Vicenin-2 inhibits the Helicobacterium pylori infection associated gastric carcinogenic events through modulation of PI3K/AKT and Nrf2 signaling in GES-1 cells.

Authors - Yifeng Zhang
mouseWB

J Ethnopharmacol

Ophiocordyceps lanpingensis polysaccharides alleviate chronic kidney disease through MAPK/NF-κB pathway.

Authors - Shubo Zhou
WB

Biomed Pharmacother

Anti-osteoporotic effects of alisol C 23-acetate via osteoclastogenesis inhibition.

Authors - Xiaokang Jia