Phospho-JNK (Tyr185) Recombinant antibody
Phospho-JNK (Tyr185) Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (3)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2G8
Cat no : 80024-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, UV treated NIH/3T3 cells, UV treated HEK-293T cells, Anisomycin treated NIH/3T3 cells, Calyculin A treated HEK-293T cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 81 publications below |
Product Information
80024-1-RR targets Phospho-JNK (Tyr185) in WB, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | mitogen-activated protein kinase 8 |
Observed molecular weight | 37-45kDa |
GenBank accession number | NM_139049 |
Gene symbol | MAPK8 |
Gene ID (NCBI) | 5599 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun.
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-JNK (Tyr185) antibody 80024-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response | ||
Br J Pharmacol Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A | ||
J Hazard Mater Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders. | ||
J Transl Med Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways | ||
Life Sci The GSTP1/MAPKs/BIM/SMAC modulatory actions of nitazoxanide: Bioinformatics and experimental evidence in subcutaneous solid Ehrlich carcinoma-inoculated mice | ||
Pharmacol Res Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways. |