Phospho-JUN (Ser73) Polyclonal antibody

Phospho-JUN (Ser73) Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat and More (2)





Cat no : 28891-1-AP


Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Phospho-JUN (Ser73), Proto oncogene c Jun, Transcription factor AP 1

Tested Applications

Positive WB detected inUV treated NIH/3T3 cells
Positive IF detected inNIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 10 publications below

Product Information

28891-1-AP targets Phospho-JUN (Ser73) in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name jun oncogene
Calculated molecular weight 331 aa, 36 kDa
Observed molecular weight38-45 kDa
GenBank accession numberBC068522
Gene symbol JUN
Gene ID (NCBI) 3725
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa.


Product Specific Protocols
WB protocol for Phospho-JUN (Ser73) antibody 28891-1-APDownload protocol
IF protocol for Phospho-JUN (Ser73) antibody 28891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Pharmacol

Activation of CXCL13/CXCR5 axis aggravates experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome.

Authors - Jiang Zhao

Int J Biol Sci

RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway.

Authors - Deyu Zhang

Front Pharmacol

Azithromycin Attenuates Bleomycin-Induced Pulmonary Fibrosis Partly by Inhibiting the Expression of LOX and LOXL-2.

Authors - Xiang Tong

J Appl Microbiol

Enterococcus faecium HDRsEf1 inhibits LPS-induced downregulation of ZO-1 expression via TLR2/4-mediated JNK/AP-1 signalling pathways.

Authors - Lin Liu

Cell Death Dis

Usp8 promotes tumor cell migration through activating the JNK pathway.

Authors - Yunhe Zhao

Cell Mol Biol Lett

Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway

Authors - Zhaoyi Liang