Phospho-MEK2 (Thr394) Polyclonal antibody

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  WB analysis using 28955-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28955-1-AP (Phospho-MEK2 (Thr394) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

Phospho-MEK2 (Thr394) Polyclonal Antibody for WB, ELISA

Cat No. 28955-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (3)

Applications

WB, IF, ELISA

Synonyms

ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, Phospho-MAP2K2 (Thr394), PRKMK2

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

28955-1-AP targets Phospho-MEK2 (Thr394) in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC018645
• Gene Symbol: MEK2
• Gene ID (NCBI): 5605
• RRID: AB_2881235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36507
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.

Publications

Species	Application	Title
mouse	WB,IF	Nat Immunol; Defining blood-induced microglia functions in neurodegeneration through multiomic profiling; Authors - Andrew S Mendiola
rat	WB	Pharm Biol; Myrtenol improves brain damage and promotes angiogenesis in rats with cerebral infarction by activating the ERK1/2 signalling pathway.; Authors - Shengming Huang
human	WB	Int J Biol Sci; Dual role of CD73 as a signaling molecule and adenosine-generating enzyme in colorectal cancer progression and immune evasion; Authors - Weidong Lian
human	WB	Cell Mol Biol Lett; A specific super-enhancer actuated by berberine regulates EGFR-mediated RAS-RAF1-MEK1/2-ERK1/2 pathway to induce nasopharyngeal carcinoma autophagy; Authors - Yao Wu
human	WB	Mol Med Rep; Downregulation of microRNA‑221‑3p promotes angiogenesis of lipoprotein(a)‑injured endothelial progenitor cells by targeting silent information regulator 1 to activate the RAF/MEK/ERK signaling pathway; Authors - Xiaolei Zhang