Tested Applications
| Positive WB detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28955-1-AP targets Phospho-MEK2 (Thr394) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase 2 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC018645 |
| Gene Symbol | MEK2 |
| Gene ID (NCBI) | 5605 |
| RRID | AB_2881235 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36507 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MEK2 (Thr394) antibody 28955-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 28955-1-AP (anti-MEK1/2) has 7 total citations across journals including Nature Immunology, Cell Mol Biol Lett, Int J Biol Sci, Pharm Biol, Mol Med Rep, Mol Cell Endocrinol, and Epilepsy Res. Research fields span neurodegeneration and microglia biology, nasopharyngeal carcinoma autophagy, colorectal cancer immune evasion, cerebral infarction angiogenesis, endothelial cell signaling, steroid hormone synthesis in granulosa cells, and epilepsy-related RAS pathway modulation. Applications include WB and IF in mouse, human, rat, and bovine species.
| Species | Application | Title |
|---|---|---|
Nat Immunol Defining blood-induced microglia functions in neurodegeneration through multiomic profiling | ||
Cell Mol Biol Lett A specific super-enhancer actuated by berberine regulates EGFR-mediated RAS-RAF1-MEK1/2-ERK1/2 pathway to induce nasopharyngeal carcinoma autophagy | ||
Int J Biol Sci Dual role of CD73 as a signaling molecule and adenosine-generating enzyme in colorectal cancer progression and immune evasion | ||
Pharm Biol Myrtenol improves brain damage and promotes angiogenesis in rats with cerebral infarction by activating the ERK1/2 signalling pathway. | ||
Mol Med Rep Downregulation of microRNA‑221‑3p promotes angiogenesis of lipoprotein(a)‑injured endothelial progenitor cells by targeting silent information regulator 1 to activate the RAF/MEK/ERK signaling pathway | ||
Mol Cell Endocrinol Copper regulates the synthesis of steroid hormones through CCS and ATOX1 dependent MEK1-ERK1/2 pathway in follicular granulosa cells of yak (Bos grunniens). |
