Phospho-MEK1 (Thr386) Recombinant antibody, PBS Only

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  WB analysis of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

  Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 81304-1-RR (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81304-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

  WB result of Phospho-MEK1 (Thr386) antibody (81304-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-MEK1 (Thr386) transfected HeLa cells. This data was developed using the same antibody clone with 81304-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-MEK1 (Thr386) antibody (81304-1-RR, Clone: 6K5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81304-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

  1X10^6 HeLa cells (dashed untreated ines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-MEK1 (Thr386) Recombinant antibody (81304-1-RR, Clone:6K5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81304-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Phospho-MEK1 (Thr386) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 81304-1-PBS

Clone No.6K5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MEK1 (Thr386), p MEK1 , p MEK1 (Thr386), p MEK1 Thr386, Phospho MEK1

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Product Information

81304-1-PBS targets Phospho-MEK1 (Thr386) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC139729
• Gene Symbol: MEK1
• Gene ID (NCBI): 5604
• ENSEMBL Gene ID: ENSG00000169032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02750
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)