Phospho-MST1 (Thr183)/MST2 (Thr180) Polyclonal antibody

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• Phospho-MST1 (Thr183)/MST2 (Thr180) Polyclonal antibody (28953-1-AP)

Phospho-MST1 (Thr183)/MST2 (Thr180) Polyclonal Antibody for WB, ELISA

Cat No. 28953-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, ELISA

Synonyms

DKFZp686A2068, KRS2, MST 1, MST1, MST1 (T183)/MST2(T180), MST2, Phospho-MST1 (T183)/MST2(T180), Phospho-MST1 (Thr183)/MST2 (Thr180), serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3

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  WB analysis using 28953-1-AP

  Non-treated Ramos and Staurosporine treated Ramos cells were subjected to SDS PAGE followed by western blot with 28953-1-AP (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Staurosporine treated Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28953-1-AP targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: serine/threonine kinase 4
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 52-56 kDa
• GenBank Accession Number: BC005231
• Gene Symbol: MST1
• Gene ID (NCBI): 6789
• RRID: AB_2918221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13043
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases. MST1/2 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the MST1/2 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. MST1 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of MST1 at Thr183 (Thr180 in MST2) in the activation loop is a key activation mechanism for MST1/2 because phosphorylation of Thr183/180 causes the cleavage of MST1 by caspases under apoptotic conditions.

Publications

What published studies show

The anti-LATS1 antibody (Cat# 28953-1-AP) has 12 total citations. Citations appear in journals including Cell Death & Disease, International Journal of Biological Sciences, Cell Reports, Chinese Medical Journal, Environmental Pollution, Biochemical Pharmacology, International Journal of Pharmaceutics, Journal of Cellular Physiology, Toxicology and Applied Pharmacology, Discover Oncology, Journal of Natural Medicine, and bioRxiv. Research fields span Hippo pathway signaling, cancer progression (bladder, glioma, lung, renal, pancreatic), apoptosis, cell proliferation, bone development, and muscle atrophy.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling; Authors - Hualin Chen
human	WB	Int J Biol Sci; S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development; Authors - Yifang Hu
mouse, human	WB	Chin Med J (Engl); GADD45B promotes apoptosis in intestinal ischemia/reperfusion through DNA demethylation of MST1/Hippo.; Authors - Fengyuan Yang
rat	WB	Cell Rep; The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells; Authors - Michael R Weaver
	WB	Environ Pollut; Microcystin-LR facilitates pubertal bone retardation by interfering with glutamine metabolism to accelerate stem cell senescence through Hippo/GLS pathway; Authors - Chun Pan
human		Biochem Pharmacol; A humanized Anti-YKL-40 antibody inhibits tumor development; Authors - Haihui Yu