Phospho-MST1 (Thr183)/MST2 (Thr180) Polyclonal antibody

Phospho-MST1 (Thr183)/MST2 (Thr180) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28953-1-AP

Synonyms

DKFZp686A2068, KRS2, MST 1, MST1, MST1 (T183)/MST2(T180), MST2, Phospho-MST1 (T183)/MST2(T180), Phospho-MST1 (Thr183)/MST2 (Thr180), serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3



Tested Applications

Positive WB detected inStaurosporine treated Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

28953-1-AP targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name serine/threonine kinase 4
Calculated molecular weight 56 kDa
Observed molecular weight52-56 kDa
GenBank accession numberBC005231
Gene symbol STK4
Gene ID (NCBI) 6789
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases. MST1/2 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the MST1/2 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. MST1 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of MST1 at Thr183 (Thr180 in MST2) in the activation loop is a key activation mechanism for MST1/2 because phosphorylation of Thr183/180 causes the cleavage of MST1 by caspases under apoptotic conditions.

Protocols

Product Specific Protocols
WB protocol for Phospho-MST1 (Thr183)/MST2 (Thr180) antibody 28953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols