Phospho-TAU (Thr181) Polyclonal antibody

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• Phospho-TAU (Thr181) Polyclonal antibody (28866-1-AP)

Phospho-TAU (Thr181) Polyclonal Antibody for WB, ELISA

Cat No. 28866-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

p Tau, p Tau (Thr181), p Tau Thr181, Phospho Tau, Phospho Tau (Thr181)

• 

  WB analysis using 28866-1-AP

  Non-treated SH-SY5Y and PDGF (HZ-1215) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28866-1-AP (Phospho-Tau (Thr181) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 28866-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28866-1-AP (Phospho-TAU (Thr181) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PDGF treated SH-SY5Y cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28866-1-AP targets Phospho-TAU (Thr181) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 37-46, 79-81 kDa
• Observed Molecular Weight: 50-80 kDa
• GenBank Accession Number: BC000558
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• RRID: AB_2881222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10636
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Basis Dis; Apolipoprotein E ε4 triggers neurotoxicity via cholesterol accumulation, acetylcholine dyshomeostasis, and PKCε mislocalization in cholinergic neuronal cells; Authors - Rebecca Piccarducci
mouse	WB	Neural Plast; Treatment Combining Focused Ultrasound with Gastrodin Alleviates Memory Deficit and Neuropathology in an Alzheimer's Disease-Like Experimental Mouse Model.; Authors - Kaixuan Luo
human	WB	Neurotoxicology; Protective mechanism of gold nanoparticles on human neural stem cells injured by β-amyloid protein through miR-21-5p/SOCS6 pathway; Authors - Guoqing Wang
mouse,human	WB	Acta Pharm Sin B; Pleiotropic prodrugs for both symptomatic and disease-modifying treatment of Alzheimer's disease.; Authors - Anže Meden
mouse	IHC	Neurochem Res; Olfactory Dysfunction Exacerbates Hippocampal Aβ Accumulation, Tau Phosphorylation and Memory Deficits in Mice.; Authors - Miaomiao Du
mouse	WB	Brain Res Bull; Induction of tau pathology and motor dysfunction in mice by urinary exosomes from progressive supranuclear palsy patients; Authors - Qiusha Li