Tested Applications
| Positive WB detected in | Serum-free treated NIH/3T3 cells, 10 % fetal bovine serum treated NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29018-1-AP targets Phospho-YAP1 (Ser397) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Yes-associated protein 1, 65kDa |
| Calculated Molecular Weight | 504 aa, 54 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC038235 |
| Gene Symbol | YAP1 |
| Gene ID (NCBI) | 10413 |
| RRID | AB_2923587 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46937 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-YAP1 (Ser397) antibody 29018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-YAP1 (Ser397) Polyclonal antibody (Cat# 29018-1-AP) has 54 citations published in journals including Bioactive Materials, Advanced Science, Cell Death & Disease, Developmental Cell, International Immunopharmacology, Phytomedicine, and Scientific Reports. Research fields encompass oncology (breast, lung, gastric, bladder, colorectal, pancreatic, liver, and skin cancers), fibrosis (hepatic, cardiac, subretinal), ferroptosis, Hippo/YAP mechanotransduction, epithelial-mesenchymal transition, neuroprotection, bone biology, and metabolic disorders. Applications are predominantly Western blot, supplemented by IHC and IF.
| Species | Application | Title |
|---|---|---|
Bioact Mater An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a "Soil-conditioning, seed-modulating, and weeds-suppressing" strategy. | ||
J Adv Res Matrix stiffness drives squamous cell carcinoma progression via a Piezo1-mediated mechanotransduction feedback loop. | ||
Adv Sci (Weinh) Identification of PKN2 and MOB4 as Coordinators of Collective Cell Migration. | ||
Adv Sci (Weinh) Tetrastigma Hemsleyanum Polysaccharide Suppresses Triple-Negative Breast Cancer by Disrupting the Hippo-YAP/TEAD4-PDIA4 Axis and Endoplasmic Reticulum Stress Adaptation. | ||
Cell Death Dis PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling | ||
Cell Death Dis CRX is an intrinsic suppressor of epithelial‒mesenchymal transition in retinal pigment epithelial cells: a promising therapeutic avenue for subretinal fibrosis. |
Reviews
What customers say
The single review for this antibody reports a 5-star rating for Western Blot on HEK293T cells at a 1:2000 dilution. The user noted it worked well to detect pYAP at 70 kDa, indicating reliable performance and clear band detection for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Udesh (Verified Customer) (08-19-2024) | Worked well to detect pYAP at 70 kDa
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