Phospho-YAP1 (Ser397) Polyclonal antibody

Phospho-YAP1 (Ser397) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse and More (1)





Cat no : 29018-1-AP


65 kDa Yes associated protein, Phospho-YAP1 (Ser397), YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog

Tested Applications

Positive WB detected inSerum-free treated NIH/3T3 cells, 10 % fetal bovine serum treated NIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29018-1-AP targets Phospho-YAP1 (Ser397) in WB,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Yes-associated protein 1, 65kDa
Calculated Molecular Weight 504 aa, 54 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC038235
Gene Symbol YAP1
Gene ID (NCBI) 10413
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)


Product Specific Protocols
WB protocol for Phospho-YAP1 (Ser397) antibody 29018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Immunol

The role of YAP1 in survival prediction, immune modulation, and drug response: A pan-cancer perspective

Authors - Xueqing Hu

Arch Biochem Biophys

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