Phospho-YAP1 (Ser397) Polyclonal antibody

Phospho-YAP1 (Ser397) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 29018-1-AP

Synonyms

65 kDa Yes associated protein, Phospho-YAP1 (Ser397), YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog

---

Validation Data Gallery

• 

  WB analysis using 29018-1-AP

  Serum-free treated NIH/3T3 cells and 10 % fetal bovine serum treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29018-1-AP (Phospho-YAP1 (Ser397) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

---

Tested Applications

• Positive WB detected in: Serum-free treated NIH/3T3 cells, 10 % fetal bovine serum treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

29018-1-AP targets Phospho-YAP1 (Ser397) in WB,ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Yes-associated protein 1, 65kDa
• Calculated Molecular Weight: 504 aa, 54 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC038235
• Gene Symbol: YAP1
• Gene ID (NCBI): 10413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)

Publications

Species	Application	Title
human	WB	Front Immunol; The role of YAP1 in survival prediction, immune modulation, and drug response: A pan-cancer perspective; Authors - Xueqing Hu
mouse	WB	Arch Biochem Biophys; Oridonin suppresses the growth of glioblastoma cells via inhibiting Hippo/YAP axis; Authors - Chen Wang
mouse,human	WB	J Nat Med; Ruscogenin ameliorates dasatinib-induced intestinal barrier dysfunction via ErbB4/YAP and ROCK/MLC pathways; Authors - Juan Huang
human	WB,IHC	Cell Death Dis; PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling; Authors - Hualin Chen
human	WB	Mol Carcinog; LATS-regulated nuclear-cytoplasmic translocation of SREBP2 inhibits hepatocellular carcinoma cell migration and invasion via epithelial-mesenchymal transition; Authors - Feng Zhang
human	WB	Biochem Biophys Res Commun; IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression; Authors - Yan Xu