Phospho-eEF2K (Ser366) Recombinant antibody, PBS Only

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  WB analysis of Jurkat using 80351-3-RR (same clone as 80351-3-PBS)

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 80351-3-RR (Phospho-eEF2K (Ser366) antibody) blocked with BSA only, Phospho-eEF2K (Ser366) peptide or non-Phospho peptide at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80351-3-PBS in a different storage buffer formulation.

Phospho-eEF2K (Ser366) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 80351-3-PBS

Clone No.250361E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

eEF2K (Ser366), eEF2K(Ser366), p eEF2K, p eEF2K (Ser366), p-eEF2K

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Product Information

80351-3-PBS targets Phospho-eEF2K (Ser366) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic elongation factor-2 kinase
• Calculated Molecular Weight: 725 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC032665
• Gene Symbol: EEF2K
• Gene ID (NCBI): 29904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00418
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic Elongation Factor-2 Kinase (eEF2K) acts as a negative regulator of protein synthesis, translation, and cell growth. As a structurally unique member of the alpha-kinase family, eEF2K is essential to cell survival under stressful conditions, as it contributes to both cell viability and proliferation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is downregulated through the phosphorylation of multiple sites via mTOR signaling and upregulated via the AMPK pathway. In the S6K-mediated pathway, RPS6KB1 or p70S6K, can phosphorylate eEF2K on Ser-366, rendering it inactive(PMID: 11500364, PMID: 34532346). The calculated molecular weight of eEF2K is 82kDa, and probably due to phosphorylation modification, resulting in a larger molecular weight, around 100kDa.