Validation Data Gallery
|Positive WB detected in||mouse brain tissue, rat brain tissue, HEK-293 cells, human brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11101-1-AP targets Rab23 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Rab23 fusion protein Ag1588|
|Full Name||RAB23, member RAS oncogene family|
|Calculated molecular weight||237 aa, 27 kDa|
|Observed molecular weight||27 kDa|
|GenBank accession number||BC015021|
|Gene ID (NCBI)||51715|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).
Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.
RAB23 coordinates early osteogenesis by repressing FGF10-pERK1/2 and GLI1.
Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway.
J Cell Sci
Differential role of Rab proteins in ciliary trafficking: Rab23 regulates smoothened levels.
Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.
Int J Biochem Cell Biol
Downregulation of Rab23 inhibits proliferation, invasion, and metastasis of human ovarian cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guorong (Verified Customer) (11-22-2022)
Multiple bands distributed at 50 and 75 kDa were observed . Does not represent the performance in other kind of samples