|Positive WB detected in||mouse brain tissue, rat brain tissue, HEK-293 cells, human brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11101-1-AP targets Rab23 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Rab23 fusion protein Ag1588|
|Full Name||RAB23, member RAS oncogene family|
|Calculated molecular weight||237 aa, 27 kDa|
|Observed molecular weight||27 kDa|
|GenBank accession number||BC015021|
|Gene ID (NCBI)||51715|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).
Am J Cancer Res
miR-338-3p targets RAB23 and suppresses tumorigenicity of prostate cancer cells.
Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics.
Int J Biochem Cell Biol
Downregulation of Rab23 inhibits proliferation, invasion, and metastasis of human ovarian cancer.
J Cell Sci
Differential role of Rab proteins in ciliary trafficking: Rab23 regulates smoothened levels.
Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway.
RAB23 coordinates early osteogenesis by repressing FGF10-pERK1/2 and GLI1.