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  • KD/KO Validated

Rab23 Antibody

Rab23 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11101-1-AP

Synonyms

HSPC137, RAB23, Ras related protein Rab 23



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, HEK-293 cells, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11101-1-AP targets Rab23 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rab23 fusion protein Ag1588
Full Name RAB23, member RAS oncogene family
Calculated molecular weight 237aa,27 kDa
Observed molecular weight 27 kDa
GenBank accession number BC015021
Gene symbol RAB23
Gene ID (NCBI) 51715
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

Protocols

Product Specific Protocols
WB protocol for Rab23 antibody 11101-1-APDownload protocol
IHC protocol for Rab23 antibody 11101-1-APDownload protocol
IP protocol for Rab23 antibody 11101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Am J Cancer Res

miR-338-3p targets RAB23 and suppresses tumorigenicity of prostate cancer cells.

Authors - Yuxiong Wang
mouseWB,IF

Development

Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics.

Authors - Hong-Hui Wang
  • KD Validated
humanWB,IHC

Int J Biochem Cell Biol

Downregulation of Rab23 inhibits proliferation, invasion, and metastasis of human ovarian cancer.

Authors - Lingling Gao
humanWB

J Cell Sci

Differential role of Rab proteins in ciliary trafficking: Rab23 regulates smoothened levels.

Authors - Boehlke Christopher C
  • KD Validated
humanWB,IF

Oncotarget

Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway.

Authors - Qiang Jian
  • KD Validated
human,mouseWB,IF

Curr Biol

Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.

Authors - Andreas Gerondopoulos
  • KD Validated