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RAB27B Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 13412-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

RAB27B Antibody WB rat spleen tissue 13412-1-AP

rat spleen tissue were subjected to SDS PAGE followed by western blot with 13412-1-AP( RAB27B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

RAB27B Antibody IHC human pancreas cancer tissue 13412-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP( RAB27B Antibody) at dilution of 1:200 (under 10x lens).

RAB27B Antibody IHC human pancreas cancer tissue 13412-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP( RAB27B Antibody) at dilution of 1:200 (under 40x lens).

RAB27B Antibody IF HeLa cells 13412-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 13412-1-AP (RAB27B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, dog
Positive WB detected in:
rat spleen tissue, A375 cells, DU 145 cells, MCF-7 cells, PC-3 cells
Positive IHC detected in:
human pancreas cancer tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human prostate cancer tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
RAB27B fusion protein Ag4064
Full name:
RAB27B, member RAS oncogene family
Calculated molecular weight:
218aa,25 kDa
Observed molecular weight:
25-30 kDa
GenBank accession number:
BC027474
Gene ID (NCBI):
5874
Gene symbol
RAB27B
Synonyms
C25KG, RAB27B, Ras related protein Rab 27B

Background

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.


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