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RAB27B Polyclonal antibody

RAB27B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 13412-1-AP


C25KG, RAB27B, Ras related protein Rab 27B

Tested Applications

Positive WB detected inrat spleen tissue, A375 cells, DU 145 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman pancreas cancer tissue, human oesophagus cancer tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-87 MG cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13412-1-AP targets RAB27B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB27B fusion protein Ag4064
Full Name RAB27B, member RAS oncogene family
Calculated Molecular Weight 218 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC027474
Gene Symbol RAB27B
Gene ID (NCBI) 5874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.


Product Specific Protocols
WB protocol for RAB27B antibody 13412-1-APDownload protocol
IHC protocol for RAB27B antibody 13412-1-APDownload protocol
IF protocol for RAB27B antibody 13412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kacie (Verified Customer) (09-12-2022)

Antibody works well for both ICC and WB.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: M17 Cells