RAB27B Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB27B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

33

Cat no : 13412-1-AP

Synonyms

C25KG, RAB27B, Ras related protein Rab 27B

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Validation Data Gallery

• 

  WB analysis of rat spleen using 13412-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13412-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13412-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13412-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 13412-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 13412-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13412-1-AP (RAB27B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: rat spleen tissue, A375 cells, DU 145 cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human pancreas cancer tissue, human oesophagus cancer tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human prostate cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 7 publications below
• WB: See 26 publications below
• IHC: See 13 publications below
• IF: See 7 publications below

Product Information

13412-1-AP targets RAB27B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB27B fusion protein Ag4064
• Full Name: RAB27B, member RAS oncogene family
• Calculated molecular weight: 218 aa, 25 kDa
• Observed molecular weight: 25-30 kDa
• GenBank accession number: BC027474
• Gene symbol: RAB27B
• Gene ID (NCBI): 5874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Publications

Species	Application	Title
human	WB	Gene; Inactivation of Rab27B-dependent signaling pathway by calycosin inhibits migration and invasion of ER-negative breast cancer cells.; Authors - Guoli Wu
human	WB,IHC	Int J Cancer; RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer.; Authors - Wei-Chung Cheng ; KD Validated
human	WB,IHC,IF	Oncotarget; Downregulation of serum RAB27B confers improved prognosis and is associated with hepatocellular carcinoma progression through PI3K-AKT-P21 signaling.; Authors - Xue Yang ; KD Validated
mouse	WB,IF	Int J Mol Sci; Inhibition of Rab27a and Rab27b Has Opposite Effects on the Regulation of Hair Cycle and Hair Growth.; Authors - Kyung-Eun Ku
human	WB,IHC,IF	J Exp Clin Cancer Res; LINC00511 drives invasive behavior in hepatocellular carcinoma by regulating exosome secretion and invadopodia formation.; Authors - Xueqiang Peng
human	WB	Cell Rep; NRF2 promotes urothelial cell response to bacterial infection by regulating reactive oxygen species and RAB27B expression.; Authors - Chetanchandra S Joshi

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