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  • KD/KO Validated

RAB27B Polyclonal antibody

RAB27B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13412-1-AP

Synonyms

C25KG, RAB27B, Ras related protein Rab 27B



Tested Applications

Positive WB detected inrat spleen tissue, A375 cells, DU 145 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13412-1-AP targets RAB27B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity dog, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB27B fusion protein Ag4064
Full Name RAB27B, member RAS oncogene family
Calculated molecular weight 218 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC027474
Gene symbol RAB27B
Gene ID (NCBI) 5874
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Protocols

Product Specific Protocols
WB protocol for RAB27B antibody 13412-1-APDownload protocol
IHC protocol for RAB27B antibody 13412-1-APDownload protocol
IF protocol for RAB27B antibody 13412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

Inactivation of Rab27B-dependent signaling pathway by calycosin inhibits migration and invasion of ER-negative breast cancer cells.

Authors - Guoli Wu
humanWB,IHC

Int J Cancer

RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer.

Authors - Wei-Chung Cheng
  • KD Validated
humanWB,IHC

Oncotarget

Downregulation of serum RAB27B confers improved prognosis and is associated with hepatocellular carcinoma progression through PI3K-AKT-P21 signaling.

Authors - Xue Yang
mouseWB,IF

Int J Mol Sci

Inhibition of Rab27a and Rab27b Has Opposite Effects on the Regulation of Hair Cycle and Hair Growth.

Authors - Kyung-Eun Ku
WB

Oncol Rep

Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion.

Authors - Hongjun Wang
dog,ratWB,IHC

J Histochem Cytochem

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.

Authors - Hiroshi Gomi