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  • KD/KO Validated

RAB2 Polyclonal antibody

RAB2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15420-1-AP

Synonyms

RAB2, RAB2A, Ras related protein Rab 2A



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, rat brain tissue
Positive IHC detected inhuman brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15420-1-AP targets RAB2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB2 fusion protein Ag7708
Full Name RAB2A, member RAS oncogene family
Calculated molecular weight24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC008929
Gene symbol RAB2A
Gene ID (NCBI) 5862
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.

Protocols

Product Specific Protocols
WB protocol for RAB2 antibody 15420-1-APDownload protocol
IHC protocol for RAB2 antibody 15420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Sci

Rab2a and Rab27a cooperatively regulate transition from granule maturation to exocytosis through the dual effector Noc2.

Authors - Kohichi Matsunaga
mouseWB

mBio

Epistatic Interplay between Type IV Secretion Effectors Engages the Small GTPase Rab2 in the Brucella Intracellular Cycle.

Authors - Erin P Smith
  • KD Validated
mouseWB

J Neurochem

Retromer and Rab2-dependent trafficking mediate PS1 degradation by proteasomes in endocytic disturbance.

Authors - Naoya Ueda
  • KD Validated
humanWB

J Hematol Oncol

Pin1 inhibition exerts potent activity against acute myeloid leukemia through blocking multiple cancer-driving pathways.

Authors - Xiaolan Lian
humanWB, IF

Cell Rep

The Rab2A GTPase promotes breast cancer stem cells and tumorigenesis via Erk signaling activation.

Authors - Man-Li Luo
  • KD Validated

EMBO Rep

RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs.

Authors - Hiroaki Kajiho