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  • KD/KO Validated

RAB2 Polyclonal antibody

RAB2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15420-1-AP

Synonyms

RAB2, RAB2A, Ras related protein Rab 2A



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, rat brain tissue
Positive IHC detected inhuman brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15420-1-AP targets RAB2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB2 fusion protein Ag7708
Full Name RAB2A, member RAS oncogene family
Calculated Molecular Weight24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC008929
Gene Symbol RAB2A
Gene ID (NCBI) 5862
RRIDAB_2176874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.

Protocols

Product Specific Protocols
WB protocol for RAB2 antibody 15420-1-APDownload protocol
IHC protocol for RAB2 antibody 15420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Authors - Shen Zhang
humanWB

Cell Host Microbe

A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.

Authors - Cheryl N Miller
  • KD Validated
humanWB

Nat Commun

Arsenic targets Pin1 and cooperates with retinoic acid to inhibit cancer-driving pathways and tumor-initiating cells.

Authors - Shingo Kozono

Proc Natl Acad Sci U S A

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.

Authors - Liang Chen

EMBO Rep

RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs.

Authors - Hiroaki Kajiho
humanWB, IF

Cell Rep

The Rab2A GTPase promotes breast cancer stem cells and tumorigenesis via Erk signaling activation.

Authors - Man-Li Luo
  • KD Validated