|Positive WB detected in||mouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, rat brain tissue|
|Positive IHC detected in||human brain tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15420-1-AP targets RAB2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB2 fusion protein Ag7708|
|Full Name||RAB2A, member RAS oncogene family|
|Calculated molecular weight||24 kDa|
|Observed molecular weight||24 kDa|
|GenBank accession number||BC008929|
|Gene ID (NCBI)||5862|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.
J Cell Sci
Rab2a and Rab27a cooperatively regulate transition from granule maturation to exocytosis through the dual effector Noc2.
Epistatic Interplay between Type IV Secretion Effectors Engages the Small GTPase Rab2 in the Brucella Intracellular Cycle.
Retromer and Rab2-dependent trafficking mediate PS1 degradation by proteasomes in endocytic disturbance.
Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells.
J Hematol Oncol
Pin1 inhibition exerts potent activity against acute myeloid leukemia through blocking multiple cancer-driving pathways.
The Rab2A GTPase promotes breast cancer stem cells and tumorigenesis via Erk signaling activation.