RACK1; GNB2L1 Polyclonal antibody

RACK1; GNB2L1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27592-1-AP

Synonyms

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1



Tested Applications

Positive WB detected inmouse liver tissue, L02 cells, HepG2 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

27592-1-AP targets RACK1; GNB2L1 in WB, IHC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity monkey, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RACK1; GNB2L1 fusion protein Ag26708
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
Calculated molecular weight 36 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC019093
Gene symbol GNB2L1
Gene ID (NCBI) 10399
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Protocols

Product Specific Protocols
WB protocol for RACK1; GNB2L1 antibody 27592-1-APDownload protocol
IHC protocol for RACK1; GNB2L1 antibody 27592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Virus Res

Integrin β3, a RACK1 interacting protein, is critical for porcine reproductive and respiratory syndrome virus infection and NF-κB activation in Marc-145 cells.

Authors - Chao Yang
monkey,pigWB

Vet Microbiol

Small molecule screening identified cepharanthine as an inhibitor of porcine reproductive and respiratory syndrome virus infection in vitro by suppressing integrins/ILK/RACK1/PKCα/NF-κB signalling axis.

Authors - Chao Yang
monkeyWB

Vet Microbiol

Inhibition of porcine reproductive and respiratory syndrome virus by PKC inhibitor dequalinium chloride in vitro.

Authors - Xiao Liu