RACK1; GNB2L1 Polyclonal antibody
RACK1; GNB2L1 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Human, mouse, rat and More (3)
WB, IP, IHC, ELISA
Cat no : 27592-1-AP
|Positive WB detected in||mouse liver tissue, L02 cells, HepG2 cells, rat liver tissue|
|Positive IHC detected in||human liver cancer tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 6 publications below|
|IP||See 1 publications below|
27592-1-AP targets RACK1; GNB2L1 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RACK1; GNB2L1 fusion protein Ag26708|
|Full Name||guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC019093|
|Gene ID (NCBI)||10399|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).
|Product Specific Protocols|
|WB protocol for RACK1; GNB2L1 antibody 27592-1-AP||Download protocol|
|IHC protocol for RACK1; GNB2L1 antibody 27592-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Ann Transl Med
USP41 promotes breast cancer via regulating RACK1.
Small molecule screening identified cepharanthine as an inhibitor of porcine reproductive and respiratory syndrome virus infection in vitro by suppressing integrins/ILK/RACK1/PKCα/NF-κB signalling axis.
Inhibition of porcine reproductive and respiratory syndrome virus by PKC inhibitor dequalinium chloride in vitro.
Integrin β3, a RACK1 interacting protein, is critical for porcine reproductive and respiratory syndrome virus infection and NF-κB activation in Marc-145 cells.
Cancer Biol Med
MIIP inhibits clear cell renal cell carcinoma proliferation and angiogenesis via negative modulation of the HIF-2α-CYR61 axis
Protein phosphatase 2A deficiency in hippocampal CA1 inhibits priming effect of morphine on conditioned place preference in mice