RACK1; GNB2L1 Polyclonal antibody

RACK1; GNB2L1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (3)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27592-1-AP

Synonyms

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1

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Validation Data Gallery

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  WB analysis using 27592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, L02 cells, HepG2 cells, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IP: See 1 publications below

Product Information

27592-1-AP targets RACK1; GNB2L1 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, monkey, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RACK1; GNB2L1 fusion protein Ag26708
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• Calculated molecular weight: 36 kDa
• Observed molecular weight: 31 kDa
• GenBank accession number: BC019093
• Gene symbol: GNB2L1
• Gene ID (NCBI): 10399
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Publications

Species	Application	Title
human	WB,IP	Ann Transl Med; USP41 promotes breast cancer via regulating RACK1.; Authors - Meiling Huang
monkey,pig	WB	Vet Microbiol; Small molecule screening identified cepharanthine as an inhibitor of porcine reproductive and respiratory syndrome virus infection in vitro by suppressing integrins/ILK/RACK1/PKCα/NF-κB signalling axis.; Authors - Chao Yang
monkey	WB	Vet Microbiol; Inhibition of porcine reproductive and respiratory syndrome virus by PKC inhibitor dequalinium chloride in vitro.; Authors - Xiao Liu
mouse	WB	Virus Res; Integrin β3, a RACK1 interacting protein, is critical for porcine reproductive and respiratory syndrome virus infection and NF-κB activation in Marc-145 cells.; Authors - Chao Yang
human	WB	Cancer Biol Med; MIIP inhibits clear cell renal cell carcinoma proliferation and angiogenesis via negative modulation of the HIF-2α-CYR61 axis; Authors - Fengqi Yan ; KD Validated
mouse	WB	Cereb Cortex; Protein phosphatase 2A deficiency in hippocampal CA1 inhibits priming effect of morphine on conditioned place preference in mice; Authors - Jing Dai