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  • KD/KO Validated

RACK1; GNB2L1 Polyclonal antibody

RACK1; GNB2L1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (3)

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 27592-1-AP

Synonyms

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1



Tested Applications

Positive WB detected inmouse liver tissue, L02 cells, HepG2 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

27592-1-AP targets RACK1; GNB2L1 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RACK1; GNB2L1 fusion protein Ag26708
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
Calculated molecular weight 36 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC019093
Gene symbol GNB2L1
Gene ID (NCBI) 10399
RRIDAB_2880917
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Protocols

Product Specific Protocols
WB protocol for RACK1; GNB2L1 antibody 27592-1-APDownload protocol
IHC protocol for RACK1; GNB2L1 antibody 27592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

J Clin Invest

CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages

Authors - Guohao Zhang
humanWB,IF,IP

J Virol

The matrix protein of respiratory syncytial virus suppresses interferon signaling via RACK1 association

Authors - Jingjing Cao
monkeyWB

Virology

RACK1 promotes porcine reproductive and respiratory syndrome virus infection in Marc-145 cells through ERK1/2 activation

Authors - Xinxian Wang
  • KD Validated
humanWB,IP

Ann Transl Med

USP41 promotes breast cancer via regulating RACK1.

Authors - Meiling Huang
monkey,pigWB

Vet Microbiol

Small molecule screening identified cepharanthine as an inhibitor of porcine reproductive and respiratory syndrome virus infection in vitro by suppressing integrins/ILK/RACK1/PKCα/NF-κB signalling axis.

Authors - Chao Yang
monkeyWB

Vet Microbiol

Inhibition of porcine reproductive and respiratory syndrome virus by PKC inhibitor dequalinium chloride in vitro.

Authors - Xiao Liu