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  • KD/KO Validated

RAD51 Polyclonal antibody

RAD51 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 14961-1-AP


BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, mouse testis tissue, K-562 cells, MCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14961-1-AP targets RAD51 in WB, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD51 fusion protein Ag6823
Full Name RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC001459
Gene Symbol RAD51
Gene ID (NCBI) 5888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.


Product Specific Protocols
WB protocol for RAD51 antibody 14961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nucleic Acids Res

SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.

Authors - Yongjuan Guan

Cell Death Differ

CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.

Authors - Yina Liao

Nucleic Acids Res

LncRNA lnc-RI regulates homologous recombination repair of DNA double-strand breaks by stabilizing RAD51 mRNA as a competitive endogenous RNA.

Authors - Liping Shen
  • KD Validated

Cell Rep

C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair

Authors - Zenan Zhou

Int J Biol Macromol

Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation

Authors - Hong-Yu Tao


BRD4 inhibition sensitizes cervical cancer to radiotherapy by attenuating DNA repair.

Authors - Mengdong Ni


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Resha (Verified Customer) (10-11-2023)

The antibody detected band at 37KDa for Rad51 protein. We used 1:1000 dilution for 20ug of protein.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells

ksh (Verified Customer) (01-17-2022)

This Antibody works for WB (1:1000) and IF (1:200).

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells