RAD51 Polyclonal antibody

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  WB analysis using 14961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14961-1-AP

  WB result of RAD51 antibody (14961-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells.

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  WB analysis of HeLa using 14961-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14961-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14961-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse thymus using 14961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 14961-1-AP (RAD51 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse thymus using 14961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 14961-1-AP (RAD51 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RAD51 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14961-1-AP

Publications(89)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, CoIP, ChIP, ELISA

Synonyms

BRCC5, HRAD51, HsRad51, HsT16930, RAD51 homolog A

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, mouse testis tissue, K-562 cells, MCF-7 cells
• Positive IHC detected in: mouse thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 73 publications below
• IHC: See 12 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

14961-1-AP targets RAD51 in WB, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD51 fusion protein Ag6823
• Full Name: RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001459
• Gene Symbol: RAD51
• Gene ID (NCBI): 5888
• RRID: AB_2177083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q06609
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao
human	WB	Nucleic Acids Res; LncRNA lnc-RI regulates homologous recombination repair of DNA double-strand breaks by stabilizing RAD51 mRNA as a competitive endogenous RNA.; Authors - Liping Shen ; KD Validated
		PLoS Biol; A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancers.; Authors - Kunming Zhao
human	WB,CoIP	Cell Rep; C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair; Authors - Zenan Zhou
human	WB	Int J Biol Macromol; Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation; Authors - Hong-Yu Tao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Resha (Verified Customer) (10-11-2023)

The antibody detected band at 37KDa for Rad51 protein. We used 1:1000 dilution for 20ug of protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

FH

ksh (Verified Customer) (01-17-2022)

This Antibody works for WB (1:1000) and IF (1:200).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells