RAD51 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAD51 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

29

Cat no : 14961-1-AP

Synonyms

BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA

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Validation Data Gallery

• 

  WB analysis using 14961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14961-1-AP

  WB result of RAD51 antibody (14961-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells.

• 

  IP experiment of mouse testis using 14961-1-AP

  IP Result of anti-RAD51 (IP:14961-1-AP, 3ug; Detection:14961-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

14961-1-AP targets RAD51 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD51 fusion protein Ag6823
• Full Name: RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• Calculated molecular weight: 37 kDa
• Observed molecular weight: 37 kDa
• GenBank accession number: BC001459
• Gene symbol: RAD51
• Gene ID (NCBI): 5888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Publications

Species	Application	Title
human	WB	Arch Biochem Biophys; MiR-34s negatively regulate homologous recombination through targeting RAD51.; Authors - Shuangjing Chen
human	WB	J Cell Mol Med; RDM1 promotes critical processes in breast cancer tumorigenesis.; Authors - Yajun Chen
human	WB	J Cell Mol Med; Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.; Authors - Xingjie Gao
human	WB	Biomed Pharmacother; Triptolide interferes with XRCC1/PARP1-mediated DNA repair and confers sensitization of triple-negative breast cancer cells to cisplatin.; Authors - Zhiwei Zhang
mouse	WB	Cell Cycle; Inhibition of survivin induces spindle disorganization, chromosome misalignment, and DNA damage during mouse embryo development.; Authors - Meng-Hao Pan
human	WB	J Exp Clin Cancer Res; UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.; Authors - Jingyuan Sun

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