RAP1GAP Polyclonal antibody

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  WB analysis using 19174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19174-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19174-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 19174-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19174-1-AP (RAP1GAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19174-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAP1GAP antibody (19174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RAP1GAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19174-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0474, RAP1 GTPase activating protein, Rap1 GTPase-activating protein 1, RAP1GA1, RAP1GAP1

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19174-1-AP targets RAP1GAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAP1GAP fusion protein Ag5576
• Full Name: RAP1 GTPase activating protein
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC054490
• Gene Symbol: RAP1GAP
• Gene ID (NCBI): 5909
• RRID: AB_2177291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P47736
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rap1 GTPase-activating protein (Rap1GAP) is an important tumor suppressor. Rap1GAP was associated with the AMPK and NF-κB signaling pathways and that Rap1GAP inhibited AMPK/SIRT1 and activated the NF-κB signaling pathway in model animals. Rap1GAP was verified to regulate the AMPK SIRT1/NF-κB signaling pathway and exacerbate the damage to myocardial cells caused by ischemia and hypoxia (PMID: 38320624). Rap1GAP is involved in Ang II-induced cardiomyocyte hypertrophy through the regulation of autophagy (PMID: 33936386). Rap1GAP, which is the GTPase-activating protein of Rap1, can bind to Rap1, promote the transformation of GTP from its active to inactive form, and terminate signal transduction (PMID: 21295042).