RAP1GAP Recombinant antibody, PBS Only

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  WB analysis using 86011-2-RR (same clone as 86011-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86011-2-RR (RAP1GAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86011-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 86011-2-RR (same clone as 86011-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86011-2-RR (RAP1GAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86011-2-PBS in a different storage buffer formulation.

RAP1GAP Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86011-2-PBS

Clone No.250422E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

KIAA0474, RAP1 GTPase activating protein, Rap1 GTPase-activating protein 1, RAP1GA1, RAP1GAP1

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Product Information

86011-2-PBS targets RAP1GAP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAP1GAP fusion protein Ag5576
• Full Name: RAP1 GTPase activating protein
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC054490
• Gene Symbol: RAP1GAP
• Gene ID (NCBI): 5909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47736
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Rap1 GTPase-activating protein (Rap1GAP) is an important tumor suppressor. Rap1GAP was associated with the AMPK and NF-κB signaling pathways and that Rap1GAP inhibited AMPK/SIRT1 and activated the NF-κB signaling pathway in model animals. Rap1GAP was verified to regulate the AMPK SIRT1/NF-κB signaling pathway and exacerbate the damage to myocardial cells caused by ischemia and hypoxia (PMID: 38320624). Rap1GAP is involved in Ang II-induced cardiomyocyte hypertrophy through the regulation of autophagy (PMID: 33936386). Rap1GAP, which is the GTPase-activating protein of Rap1, can bind to Rap1, promote the transformation of GTP from its active to inactive form, and terminate signal transduction (PMID: 21295042).