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RBBP5 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, ChIP-Seq, WB

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Cat No : 61405,61406 61405

Synonyms

RBBP5, retinoblastoma binding protein 5, Retinoblastoma-binding protein RBQ-3, RBQ3, RBBP-5, SWD1, SWD1, Set1c WD40 repeat protein, homolog, retinoblastoma-binding protein 5, polyclonal, pAb, antibody, western blotting, wb, sample, trithorax, trxg, trx

Validation Data Gallery

  • RBBP5 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of human PBMC chromatin and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 21 million sequence tags were mapped to identify RBBP5 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

    RBBP5 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of human PBMC chromatin and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 21 million sequence tags were mapped to identify RBBP5 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

  • RBBP5 antibody (pAb) tested by Western blot. MCF-7 whole-cell extract (20 ug) probed with RBBP5 antibody at a dilution of 1:1,000.

    RBBP5 antibody (pAb) tested by Western blot. MCF-7 whole-cell extract (20 ug) probed with RBBP5 antibody at a dilution of 1:1,000.

Product Information

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP: 10 ul per ChIP ChIP-Seq: 10 ul each WB: 1:500 - 1:5,000 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the C-terminal region of human RBBP5.
Full Name RBBP5 antibody (pAb)
Synonyms RBBP5, retinoblastoma binding protein 5, Retinoblastoma-binding protein RBQ-3, RBQ3, RBBP-5, SWD1, SWD1, Set1c WD40 repeat protein, homolog, retinoblastoma-binding protein 5, polyclonal, pAb, antibody, western blotting, wb, sample, trithorax, trxg, trx
Molecular weight 70 kDa
GenBank accession numberNP_005048
RRIDAB_2793622
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

As part of the MLL1/MLL complex, RBBP5 (retinoblastoma binding protein 5) is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.