|Positive WB detected in||Raji cells, A431 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
12624-1-AP targets RBBP8 in WB,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||RBBP8 fusion protein Ag3314|
|Full Name||retinoblastoma binding protein 8|
|Calculated molecular weight||103 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC030590|
|Gene ID (NCBI)||5932|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RBBP8 is an endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. It functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. It interacted with BRCA1 to form a complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Also it promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations
Cell Biochem Funct
The CTIP-mediated repair of TNF-α-induced DNA double-strand break was impaired by miR-130b in cervical cancer cell.
RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.