RBM12 Polyclonal antibody

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  WB analysis using 30990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30990-1-AP (RBM12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 30990-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30990-1-AP (RBM12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 30990-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM12 antibody (30990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RBM12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 30990-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RNA-binding protein 12, RNA-binding motif protein 12, RNA binding protein 12, RNA binding motif protein 12, KIAA0765

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Tested Applications

• Positive WB detected in: MOLT-4 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30990-1-AP targets RBM12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM12 fusion protein Ag34621
• Full Name: RNA binding motif protein 12
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC013981
• Gene Symbol: RBM12
• Gene ID (NCBI): 10137
• RRID: AB_3669810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NTZ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA binding motif protein 12 (RBM12) is a member of the RBM family. RBM proteins are involved in RNA metabolism, including splicing, transport, translation and stability. RBM12 has been identified as a key protein that increases the expression of PD-L1, thereby promoting immune evasion in hepatocellular carcinoma (PMID: 39187545).