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  • KD/KO Validated

RBP4 Polyclonal antibody

RBP4 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11774-1-AP

Synonyms

Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4



Tested Applications

Positive WB detected inhuman plasma tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11774-1-AP targets RBP4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Camelus bactrianus, geese, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBP4 fusion protein Ag2448
Full Name retinol binding protein 4, plasma
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC020633
Gene symbol RBP4
Gene ID (NCBI) 5950
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

Protocols

Product Specific Protocols
WB protocol for RBP4 antibody 11774-1-APDownload protocol
IHC protocol for RBP4 antibody 11774-1-APDownload protocol
IF protocol for RBP4 antibody 11774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Int J Mol Sci

Retinoid Regulation of Ocular Surface Innate Inflammation.

Authors - Jehan Alam
geeseWB

Poult Sci

Comparative proteomic analysis of ovaries from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach.

Authors - Z Cao
Camelus bactrianusWB

Reprod Domest Anim

Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes.

Authors - Qi Wang
humanWB

Proteome Sci

Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography.

Authors - Lu Chih-Ming CM
mouseWB,IHC

Reprod Biol Endocrinol

Regulation by FSH of the dynamic expression of retinol-binding protein 4 in the mouse ovary.

Authors - Yanwen Jiang
mouseWB

Lab Invest

Severe ocular phenotypes in Rbp4-deficient mice in the C57BL/6 genetic background.

Authors - Jingling Shen
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (05-31-2021)

Human spinal cord (cervical) tissue In 1/100 dilution gave fade labelling

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Human spinal cord (cervical) tissue
RBP4 Antibody Immunohistochemistry validation (1/100 dilution) in Human spinal cord (cervical) tissue (Cat no:11774-1-AP)