|Positive WB detected in||human plasma tissue, human liver tissue, mouse liver tissue, rat liver tissue|
|Positive IHC detected in||human liver cancer tissue, mouse liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse eye tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11774-1-AP targets RBP4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Camelus bactrianus, geese, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RBP4 fusion protein Ag2448|
|Full Name||retinol binding protein 4, plasma|
|Calculated molecular weight||201 aa, 23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC020633|
|Gene ID (NCBI)||5950|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)
Int J Mol Sci
Retinoid Regulation of Ocular Surface Innate Inflammation.
Comparative proteomic analysis of ovaries from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach.
Reprod Domest Anim
Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes.
Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography.
Reprod Biol Endocrinol
Regulation by FSH of the dynamic expression of retinol-binding protein 4 in the mouse ovary.
Severe ocular phenotypes in Rbp4-deficient mice in the C57BL/6 genetic background.