RBPJ antibody (mAb) (Clone 7A11)
Host / Isotype
Rat / IgG2b
Reactivity
Human
Applications
WB
CloneNo.
7A11
Cat No : 61073,61074 61073
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 2 - 4 ug/ml dilution |
| Tested Reactivity | Human |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This antibody was raised against full-length recombinant human RBPJ protein. |
| Full Name | RBPJ antibody (mAb) (Clone 7A11) |
| Synonyms | CBF-1, cbf1, RBPJk, RBPJ1, Supressor of hairless, SUH, transcriptional, Notch, monoclonal, clone 7A11, sample |
| Molecular weight | 55 kDa |
| GenBank accession number | NP_005340 |
| RRID | AB_2793496 |
| Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.